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    Mouse Anti-SMAD4 Monoclonal Antibody (clone 4G1C6) (CAT#: VS7-0425-WR879)

    This product is a mouse IgG1 antibody that binds to human SMAD4. The antibody 4G1C6 can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse.

    Specific Inquiry
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    • Purity:

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human rectum
    Figure 2 IHC staining of human rectum
    (Immunohistochemical staining of human rectum shows strong positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_9_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_6_6.jpg
    Figure 8 Placenta
    Figure 8 Placenta
    (Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_2_7.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141646-SMAD4

    Specifications

    • Immunogen
    • Recombinant human SMAD4 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human SMAD4
    • Species Reactivity
    • Human, Mouse
    • Clone
    • 4G1C6
    • Molecule Class
    • Smad Family
    • Applications
    • WB, IHC, ICC, FC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Pancreatic cancer (PNCA); Juvenile polyposis syndrome (JPS); Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT); Colorectal cancer (CRC); Myhre syndrome (MYHRS)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Immunocytochemistry: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • SMAD4; JIP; Deleted In Pancreatic Carcinoma Locus 4; Smad4; SMAD 4; Mothers Against DPP Homolog 4; SMAD, Mothers Against DPP Homolog 4; HSMAD4; Mothers Against Decapentaplegic, Drosophila, Homolog Of, 4; SMAD, Mothers Against DPP Homolog 4 (Drosophila); D
    • Long Name
    • SMAD Family Member 4
    • Cellular Localization
    • Cytoplasm; Nucleus
    • Post Translation Modifications
    • Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
    • Function
    • DNA-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "SMAD4"

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    CAT Product Name Application Type
    MOB-2804z Mouse Anti-SMAD4 Recombinant Antibody (clone 22G1) WB, ELISA, FC, IF, IHC Mouse IgG
    MRO-1405-CN Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (CBACN-513) WB, IF, IHC, FC Rabbit IgG
    MRO-2194-CN Rabbit Anti-SMAD4 Polyclonal Antibody (MRO-2194-CN) IF, IHC, FC Rabbit IgG
    VS3-QX1028 Mouse Anti-SMAD4 Recombinant Antibody (clone 5B8-F7-F3) WB, IHC, ICC, FC, ELISA Mouse IgG1
    VS3-FY1359 Recombinant Rabbit Anti-SMAD4 Antibody (clone R05-1I7) WB, ICC, IF, IP Rabbit IgG

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