Rabbit Anti-SMAD4 Recombinant Antibody (VS3-WK935)
CAT#: VS3-WK935
This product is a rabbit monoclonal antibody that recognizes SMAD4.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_6_6.jpg
Normal Tissue
Figure 8 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_2_7.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141646-SMAD4
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Derivation
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SMAD4
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 1*TBS (pH 7.4), 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at -20°C for 1 year.Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Flow Cytometry.
Target
- Alternative Names
- SMAD Family Member 4; Deletion Target In Pancreatic Carcinoma 4; MAD Homolog 4; MADH4; DPC4; MAD; Mothers Against Decapentaplegic Homolog 4 (Drosophila); Mothers Against Decapentaplegic; Drosophila; Homolog Of; 4; SMAD; Mothers Against DPP Homolog 4 (Drosophila); Mothers Against Decapentaplegic Homolog 4
- Gene ID
- 4089
- UniProt ID
- Q13485
- Long Name
- SMAD Family Member 4
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Related Diseases
Colorectal Cancer
Gastric Cancer
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
See other products for "SMAD4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3323 | Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (DS3323AB) | WB, IHC-P, ICC, FC, IP | IgG |
| MOR-4736 | Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (TH250DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1405-CN | Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (CBACN-513) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2194-CN | Rabbit Anti-SMAD4 Polyclonal Antibody (MRO-2194-CN) | IF, IHC, FC | Rabbit IgG |
| VS4-CJ119 | Recombinant Rabbit Anti-SMAD4 Antibody (clone 22H12) | ELISA, WB | Rabbit IgG |
| VS7-0425-WR879 | Mouse Anti-SMAD4 Monoclonal Antibody (clone 4G1C6) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR880 | Mouse Anti-SMAD4 Monoclonal Antibody (VS7-0425-WR880) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC832 | AbPlus™ Anti-SMAD4 Magnetic Beads (VS-0724-YC832) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY201 | Human Anti-SMAD4 scFv-Fc Chimera (VS-0325-FY201) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2107 | Anti-SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6721 | Anti-Mouse SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6720 | Anti-Human SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6722 | Anti-Rat SMAD4 Immunohistochemistry Kit | IHC |
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