Mouse Anti-SMARCA4 Recombinant Antibody (ZG-0350F)
CAT#: ZG-0350F
This product is a mouse antibody that recognizes SMARCA4.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-SMARCA4 Recombinant Antibody (ZG-0350F) in WB.
The BRG1 mouse mAb (1:1000 dilution) was used to detect BRG1 in Hela NE, F9 and K562 cell lysates by Western blot. Predicted band size: 220KDa. Observed band size: 220KDa.
IF
Figure 2 Mouse Anti-SMARCA4 Recombinant Antibody (ZG-0350F) in IF.
Immunofluorescence analysis was performed on Hela cells fixed with 4% paraformaldehyde and using anti-BRG1 mouse mAb (diluted 1:50). DAPI is used to stain the nucleus (blue).
IP
Figure 3 Mouse Anti-SMARCA4 Recombinant Antibody (ZG-0350F) in IP.
The BRG1 mouse mAb (201025) was used for immunoprecipitation analysis of K562 cell lysates.
❮
❯
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48340/831_G7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/659_G5_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000127616-SMARCA4
❮
❯
Specifications
- Immunogen
- Recombinant human BRG1 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SMARCA4
- Species Reactivity
- Human, Mouse
- Applications
- Western Blot, Immunocytochemistry
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Immunocytochemistry: 1:50
Target
- Alternative Names
- SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4; Mitotic Growth And Transcription Activator; ATP-Dependent Helicase SMARCA4; Global Transcription Activator Homologous Sequence; Sucrose Nonfermenting-Like 4; BRG1-Associated Factor 190A; Protein Brahma Homolog 1; BRM/SWI2-Related Gene 1; Homeotic Gene Regulator; Brahma Protein-Like 1; Nuclear Protein GRB1; Protein BRG-1; SNF2-Like 4; SNF2-Beta; BAF190A; SNF2L4; ATP dependent helicase SMARCA4; ATP-dependent helicase SMARCA4; BAF 190; BAF190; BAF190A; Brahma protein homolog 1; Brahma protein like 1; BRG1; BRG1 associated factor 190A; BRG1 protein; BRG1-associated factor 190A; BRM/SWI2 related gene 1; Global transcription activator homologous sequence; global transcription activator snf2l4; Homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; MRD16; Nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; Protein BRG1; RTPS2; SMARC A4; SMARCA4; SMCA4_HUMAN; SNF2; SNF2 beta; SNF2 like 4; SNF2- beta; SNF2B; SNF2L4; SNF2LB; Sucrose nonfermenting like 4; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SWI2; Transcription activator BRG1.
- Gene ID
- 6597
- UniProt ID
- P51532
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0350F. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "SMARCA4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-546 | Recombinant Human Anti-Human SMARCA4 Antibody Fab Fragment, clone D290 | IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1488MZ | Recombinant Mouse Anti-Human SMARCA4 Antibody (clone CSH-02) | WB | Mouse antibody |
| ZG-0348F | Mouse Anti-SMARCA4 Recombinant Antibody (ZG-0348F) | WB | Mouse IgG |
| ZG-0349F | Mouse Anti-SMARCA4 Recombinant Antibody (ZG-0349F) | WB | Mouse IgG |
| ZG-433C | Human Anti-SMARCA4 Recombinant Antibody (ZG-433C) | ELISA, IP | Human IgG1, κ |
| VS3-FY1361 | Recombinant Rabbit Anti-Smarca4 Antibody (clone R07-4B7) | WB, IHC-Fr, IHC-P, ICC, IF, IP, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3328 | Hi-Affi™ Recombinant Rabbit Anti-SMARCA4 Monoclonal Antibody (DS3328AB) | ICC, IF, IHC-P, WB | IgG |
| MOR-0080-FY | Rabbit Anti-SMARCA4 Recombinant Antibody (clone AFY0051) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY239 | AbPlus™ Anti-SMARCA4 Magnetic Beads (D503) | IP-MS, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2113 | Anti-SMARCA4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6731 | Anti-Mouse SMARCA4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6730 | Anti-Human SMARCA4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6732 | Anti-Rat SMARCA4 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: WB, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA
Application: Neut, ELISA, WB, SPR, IF, EM
Application: ELISA, WB, Microarray, Block
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
