Rabbit Anti-SMARCB1 Recombinant Antibody (clone R06-2C2) (CAT#: VS3-XY3509)
It is a recombinant rabbit antibody targeting the SMARCB1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1), which plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. The antibody specifically reacts with human, mouse and rat SMARCB1. It has been determined using Western Blot and Immunoprecipitation. The recommended dilutions of Western Blot and Immunoprecipitation is 1/500-1/1000 and 1/20, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/144_B9_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/144_B9_2_selected.jpg
Figure 2 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_8_selected.jpg
(Immunohistochemical staining of human tonsil shows nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_8_selected.jpg
Figure 3 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_6.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_8.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_B_8_5.jpg
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_3.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_5.jpg
Figure 8 Urinary bladder
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_5.jpg
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_5.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099956-SMARCB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099956-SMARCB1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SMARCB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-2C2
- Applications
- WB, IP
- Related Disease
- Coffin-Siris Syndrome 3; Rhabdoid Tumor Predisposition Syndrome 1
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- RDT; CSS3; INI1; SNF5; Snr1; BAF47; INI-1; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
- Gene ID
- 6598
- UniProt ID
- Q12824
- Long Name
- SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys8, Lys292, and Lys324 (NX_Q12824 [NX_Q12824-1])
Modification sites at PhosphoSitePlus (Q12824)
Glycosylation from GlyGen (Q12824) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001304875.1
- Function
- Core component of the BAF (hSWI/SNF) complex.
This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation.
The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal.
This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers.
Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4029z | Mouse Anti-SMARCB1 Recombinant Antibody (clone 21H6) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1665MZ | Recombinant Mouse Anti-Human SMARCB1 Antibody | IHC-P, WB | Mouse antibody |
| MOB-0057F | Mouse Anti-SMARCB1 Recombinant Antibody (MOB-0057F) | IHC-P, IF | Mouse IgG |
| VS3-WK908 | Mouse Anti-SMARCB1 Recombinant Antibody (clone ABT-INI1) | IHC-P, WB | Mouse IgG |
| VS3-FY1362 | Recombinant Mouse Anti-SMARCB1 Antibody (clone 5F1-6F6-3A1) | IHC-P | Mouse IgG |
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