Rabbit Anti-SMC1A (pS957) Recombinant Antibody (clone R09-9X2)
CAT#: VS3-WK2134
This product is a rabbit recombinant antibody that is specific to phosphorylated SMC1A. The target of this antibody is phospho-SMC1A (Ser957). The clone R09-9X2 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, and IHC staining at a dilution of 1/50-1/100. The molecular weight of SMC1A (pSer957) observed in WB experiments using this antibody is 160 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_8_5.jpg
Normal Tissue
Figure 6 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_4_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000072501-SMC1A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser957 of human SMC1A
- Species Reactivity
- Human
- Clone
- R09-9X2
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- Structural Maintenance Of Chromosomes 1A; SMC1 (Structural Maintenance Of Chromosomes 1, Yeast)-Like 1; SMC Protein 1A; SMC-1-Alpha; DXS423E; SMC1L1; SB1.8; SMC1; SMC1 Structural Maintenance Of Chromosomes 1-Like 1 (Yeast)
- Gene ID
- 8243
- UniProt ID
- Q14683
- Long Name
- Structural Maintenance Of Chromosomes 1A
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "SMC1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2844z | Mouse Anti-SMC1A Recombinant Antibody (clone 26G2) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-097R | Mouse Anti-SMC1A Recombinant Antibody (ZG-097R) | WB | Mouse IgG |
| ZG-098R | Mouse Anti-SMC1A Recombinant Antibody (ZG-098R) | WB, ICC | Mouse IgG |
| VS3-QX1032 | Mouse Anti-SMC1A Recombinant Antibody (clone 8E6-A4-F3) | WB, IHC, FC | Mouse IgG1 |
| VS4-CJ121 | Recombinant Mouse Anti-SMC1A Antibody (VS4-CJ121) | ELISA, WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3335 | Hi-Affi™ Recombinant Rabbit Anti-SMC1A Monoclonal Antibody (DS3335AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2119 | Anti-SMC1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY6741 | Anti-Mouse SMC1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY6740 | Anti-Human SMC1A Immunohistochemistry Kit | IHC |
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