Mouse Anti-SMN1 Monoclonal Antibody (clone 5H1)
CAT#: VS7-0425-WR892
This product is a mouse IgG1 antibody that binds to human SMN1. The antibody 5H1 can be used for WB, IHC, and ICC assays. It cross reacts with monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16324/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69888/151521_A_4_4_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73601/1729_C10_3_selected.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_6_6.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_8_3.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_8_8.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_9_5.jpg
Normal Tissue
Figure 8 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_B_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (MOLT-4, NB-4, T-47d, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172062-SMN1
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Specifications
- Immunogen
- Recombinant human SMN1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SMN1
- Species Reactivity
- Human, Monkey
- Clone
- 5H1
- Molecule Class
- Survival Motor Neuron Protein
- Applications
- WB, IHC, ICC
- Conjugate
- Unconjugated
- Related Disease
- Spinal muscular atrophy 1 (SMA1); Spinal muscular atrophy 2 (SMA2); Spinal muscular atrophy 3 (SMA3); Spinal muscular atrophy 4 (SMA4)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Survival Of Motor Neuron 1, Telomeric; Gemin-1; Tudor Domain Containing 16A; Component Of Gems 1; SMNT; SMN; Spinal Muscular Atrophy (Werdnig-Hoffmann Disease, Kugelberg-Welander Disease); Survival Motor Neuron 1 Protein; Survival Motor Neuron Protein; SMN1 SMN2; T-BCD541
- Gene ID
- 6606
- UniProt ID
- Q16637
- Long Name
- Survival Of Motor Neuron 1, Telomeric
- Cellular Localization
- Cell projection; Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000335.1
- Function
- RNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SMN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3342 | Hi-Affi™ Recombinant Rabbit Anti-SMN1 Monoclonal Antibody (DS3342AB) | WB, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
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| ZG-100R | Mouse Anti-SMN1 Recombinant Antibody (ZG-100R) | WB, ELISA, IHC, IF | Mouse IgG |
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| VS3-WK479 | Rabbit Anti-SMN1 Recombinant Antibody (clone JG38-19) | WB, ELISA | Rabbit IgG |
| VS3-FY1363 | Recombinant Rabbit Anti-SMN1 Antibody (clone R05-3B5) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY486 | Mouse Anti-NHP SMN1 Recombinant Antibody (clone 2F1) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6750 | Anti-SMN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6751 | Anti-Mouse SMN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6752 | Anti-Rat SMN1 Immunohistochemistry Kit | IHC |
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