Rabbit Anti-SNX1 Recombinant Antibody (clone R02-0A4)
CAT#: VS3-FY2089
This R02-0A4 recombinant monoclonal antibody demonstrated capability to bind to human SNX1. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The SNX1 antibody is recommended for detection of target protein by western blot analysis of SNX1 in Hela, A549, HL-60, U2OS, U87-MG lysates and immunocytochemistry analysis of SNX1 (green) in hela using SNX1 antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to endosomes & lysosomes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47373/786_A11_9_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52761/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47373/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47373/109835_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47373/110363_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47373/110363_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47373/110363_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000028528-SNX1
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WB
Figure 1 Anti-SNX1 Antibody (VS3-FY2089) in WB.
The SNX1 expression in Hela, A549, HL-60, U2OS, U87-MG lysates were detected using the anti-SNX1 by western blot assay.
ICC
Figure 2 Anti-SNX1 Antibody (VS3-FY2089) in ICC.
Immunocytochemistry staining of SNX1 (green) in hela using the anti-SNX1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human SNX1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SNX1
- Species Reactivity
- Human
- Clone
- R02-0A4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Parkinson Disease, Late-Onset and Hereditary Spastic Paraplegia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Sorting Nexin 1; Sorting Nexin 1A; Sorting Nexin-1; HsT17379; VPS5
- Gene ID
- 6642
- UniProt ID
- Q13596
- Sequence Similarities
- Belongs to the sorting nexin family.
- Cellular Localization
- Cell projection, Endosome, Golgi apparatus, Membrane
- Post Translation Modifications
- Ubiquitination at Lys286 (NX_Q13596 [NX_Q13596-1])
Modification sites at PhosphoSitePlus (Q13596)
Glycosylation from GlyGen (Q13596) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001229862.1; NP_003090.2; NP_683758.1
- Function
- Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SNX1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1667MZ | Recombinant Mouse Anti-Human SNX1 Antibody | IHC-P, WB | Mouse antibody |
| MRO-1416-CN | Recombinant Rabbit Anti-SNX1 Monoclonal Antibody (JG40-06) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK501 | Rabbit Anti-SNX1 Recombinant Antibody (clone JG40-06) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0545MZ | Chicken Anti-SNX1 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0546MZ | Chicken Anti-SNX1 (ab2) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3362 | Hi-Affi™ Recombinant Rabbit Anti-SNX1 Monoclonal Antibody (DS3362AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6787 | Anti-SNX1 Immunohistochemistry Kit | IHC |
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