Rabbit Anti-SOD1 Recombinant Antibody (VS3-FY2877)
CAT#: VS3-FY2877
The anti-SOD1 antibody is a rabbit recombinant monoclonal antibody. It has been specifically designed to target the human SOD1 protein. It cross-reacts with mouse, rat species. This monoclonal antibody proves valuable in disease research related to Spastic Tetraplegia And Axial Hypotonia, Progressive and Amyotrophic Lateral Sclerosis 1. This monoclonal antibody is suitable for employment in Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8670/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/30_D2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/29_D2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/31_D2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142168-SOD1
❮
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SOD1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated, FITC
- Related Disease
- Spastic Tetraplegia And Axial Hypotonia, Progressive and Amyotrophic Lateral Sclerosis 1
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Superoxide Dismutase 1; Superoxide Dismutase 1, Soluble; EC 1.15.1.1; HSod1; Amyotrophic Lateral Sclerosis 1 (Adult); Epididymis Secretory Protein Li 44; Superoxide Dismutase, Cystolic; Superoxide Dismutase [Cu-Zn]; Cu/Zn Superoxide Dismutase
- Gene ID
- 6647
- UniProt ID
- P00441
- Sequence Similarities
- Belongs to the Cu-Zn superoxide dismutase family.
- Cellular Localization
- Cytoplasm
Mitochondrion
Nucleus
- Post Translation Modifications
- Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation.
The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
(P00441-SODC_HUMAN)
The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization.
Such modification might only occur in extreme conditions and additional experimental evidence is required.
(P00441-SODC_HUMAN)
Palmitoylation helps nuclear targeting and decreases catalytic activity. (P00441-SODC_HUMAN)
Succinylation, adjacent to copper catalytic site, probably inhibits activity.
Desuccinylation by SIRT5 enhances activity.
(P00441-SODC_HUMAN)
Glycosylation at Asn87 (NX_P00441 [NX_P00441-1])
Ubiquitination at Lys10, Lys31, and Lys123 (NX_P00441 [NX_P00441-1])
Modification sites at PhosphoSitePlus (P00441)
Glycosylation from GlyConnect SODC_HUMAN (1778)
Glycosylation from GlyGen (P00441) 2 sites, 2 N-linked glycans (1 site), 1 O-linked glycan (1 site)
- Function
- Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
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Related Diseases
Amyotrophic Lateral Sclerosis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SOD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-465CT | Anti-Human SOD1 Single Domain Antibody (TAB-465CT), Research Grade Biosimilar | ELISA, WB, IHC, IP | Single domain antibody |
| MD-ZBF-YC020 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC020) | WB, IP, IHC, ELISA | Camelid VHH |
| MD-ZBF-YC021 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC021) | WB, IP, IHC, ELISA | Camelid VHH |
| MD-ZBF-YC022 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC022) | WB, IP, IHC, ELISA | Camelid VHH |
| MD-ZBF-YC023 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC023) | WB, IP, IHC, ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-466CT | Mouse Anti-SOD1 Recombinant Antibody (TAB-466CT) | WB | Mouse IgG |
| TAB-467CT | Mouse Anti-SOD1 Recombinant Antibody (TAB-467CT) | WB, IF | Mouse IgG |
| TAB-466CT-F(E) | Mouse Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-466CT-F(E)) | ELISA, WB, BL, IHC | Mouse Fab |
| TAB-467CT-F(E) | Mouse Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-467CT-F(E)) | ELISA, WB, BL, IHC | Mouse Fab |
| TAB-468CT-F(E) | Mouse Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-468CT-F(E)) | ELISA, WB, BL, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-469CT-F(E) | Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-469CT-F(E)) | ELISA | Human Fab |
| TAB-470CT-F(E) | Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-470CT-F(E)) | ELISA | Human Fab |
| TAB-471CT-F(E) | Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-471CT-F(E)) | ELISA | Human Fab |
| TAB-472CT-F(E) | Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-472CT-F(E)) | ELISA | Human Fab |
| TAB-473CT-F(E) | Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-473CT-F(E)) | IF, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3369 | Hi-Affi™ Recombinant Rabbit Anti-SOD1 Monoclonal Antibody (DS3369AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2196-CN | Rabbit Anti-SOD1 Polyclonal Antibody (MRO-2196-CN) | WB, IF, FC | Rabbit IgG |
| ZG-0574U | Rabbit Anti-SOD1 Recombinant Antibody (clone 3E5) | ELISA, WB, FC | Rabbit IgG |
| VS3-XY1451 | Mouse Anti-SOD1 Recombinant Antibody (clone 6F5) | ELISA, WB, FC, ICC | Mouse IgG1 |
| VS-0923-FY138 | Recombinant Mouse Anti-SOD1 Antibody (VS-0923-FY138) | ELISA, WB | Mouse IgG1 |
| VS7-0425-WR905 | Mouse Anti-SOD1 Monoclonal Antibody (VS7-0425-WR905) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY245 | AbPlus™ Anti-SOD1 Magnetic Beads (VS-0424-XY245) | IP, Protein Purification | |
| VS-0724-YC1461 | AbPlus™ Anti-SOD1 Magnetic Beads (VS-0724-YC1461) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1092 | CytoStream™ Rabbit Anti-SOD1 Recombinant Antibody (VS13-YC1092) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2141 | Anti-SOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6803 | Anti-Mouse SOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6802 | Anti-Human SOD1 Immunohistochemistry Kit | IHC |
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