Mouse Anti-SOD2 Recombinant Antibody (clone 8H3D2)
CAT#: VS7-0425-WR906
This product is a mouse IgG1 antibody that binds to human SOD2. The antibody 8H3D2 recognizes specific amino acid domain of human SOD2 (AA: 1-222). It can be used for IHC, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1814/1865_C5_1_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_6_6.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_8_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, GAMG, HSkMC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112096-SOD2
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Specifications
- Immunogen
- Recombinant human SOD2 (AA: 1-222) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SOD2
- Species Reactivity
- Human
- Clone
- 8H3D2
- Molecule Class
- Superoxide Dismutase
- Applications
- IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Microvascular complications of diabetes 6 (MVCD6)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:50 - 1:250
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SOD2; Manganese-Containing Superoxide Dismutase; EC 1.15.1.1; MNSOD; IPOB; Mangano-Superoxide Dismutase; Superoxide Dismutase 2, Mitochondrial; Mn Superoxide Dismutase
- Gene ID
- 6648
- UniProt ID
- P04179
- Long Name
- Superoxide Dismutase 2
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation; Nitration; Ubl conjugation
- Protein Refseq
- NP_000627.2
- Function
- Oxidoreductase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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