Mouse Anti-SOD2 Recombinant Antibody (clone 8H3F9) (CAT#: VS7-0425-WR907)
This product is a mouse IgG1 antibody that binds to human SOD2. The antibody 8H3F9 recognizes specific amino acid domain of human SOD2 (AA: 1-222). It can be used for WB, IHC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1814/1865_C5_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1814/1865_C5_1_selected.jpg
Figure 2 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_6_6.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_8_8.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_9_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_7_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, GAMG, HSkMC)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112096-SOD2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112096-SOD2
Specifications
- Immunogen
- Recombinant human SOD2 (AA: 1-222) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SOD2
- Species Reactivity
- Human
- Clone
- 8H3F9
- Molecule Class
- Superoxide Dismutase
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Microvascular complications of diabetes 6 (MVCD6)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SOD2; Manganese-Containing Superoxide Dismutase; EC 1.15.1.1; MNSOD; IPOB; Mangano-Superoxide Dismutase; Superoxide Dismutase 2, Mitochondrial; Mn Superoxide Dismutase
- Gene ID
- 6653
- UniProt ID
- P04179
- Long Name
- Superoxide Dismutase 2
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation; Nitration; Ubl conjugation
- Protein Refseq
- NP_000627.2
- Function
- Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3370 | Hi-Affi™ Recombinant Rabbit Anti-SOD2 Monoclonal Antibody (DS3370AB) | WB, IHC, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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