Mouse Anti-SOD2 Recombinant Antibody (VS3-CJ334)
CAT#: VS3-CJ334
This product is a mouse antibody that recognizes SOD2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1814/1865_C5_1_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_6_6.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_8_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, GAMG, HSkMC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112096-SOD2
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Specifications
- Immunogen
- Recombinant protein of human SODM
- Host Species
- Mouse
- Specificity
- Human and Mouse SOD2
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:2000), Immunohistochemistry (1:25), Flow Cytometry (1:25).
Target
- Alternative Names
- GC1; IPOB; IPO-B; MNSOD; MVCD6; GClnc1; Mn-SOD
- Gene ID
- 6648
- UniProt ID
- P04179
- Sequence Similarities
- Belongs to the iron/manganese superoxide dismutase family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity).
Polyubiquitinated; leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.
- Protein Refseq
- NP_000627.2; NP_001019636.1; NP_001019637.1
- Function
- Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SOD2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1985CT | Recombinant Mouse anti-Human SOD2 Monoclonal antibody (2BF) | ELISA, IP | |
| VS3-QX1040 | Mouse Anti-SOD2 Recombinant Antibody (clone 4C4-G8-H12) | WB | Mouse IgG2b |
| VS3-QX1041 | Mouse Anti-SOD2 Recombinant Antibody (clone 37CT127.5.11.6) | WB, IHC | Mouse IgG1, κ |
| VS7-0425-WR906 | Mouse Anti-SOD2 Recombinant Antibody (clone 8H3D2) | IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR907 | Mouse Anti-SOD2 Recombinant Antibody (clone 8H3F9) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3370 | Hi-Affi™ Recombinant Rabbit Anti-SOD2 Monoclonal Antibody (DS3370AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC500 | AbPlus™ Anti-SOD2 Magnetic Beads (VS-0724-YC500) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2142 | Anti-SOD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6805 | Anti-Rat SOD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6804 | Anti-Human SOD2 Immunohistochemistry Kit | IHC |
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