Rabbit Anti-SOX2 Recombinant Antibody (clone 2E1) (CAT#: ZG-0015U)
The purified Rabbit Anti-SOX2 Recombinant Antibody (clone 2E1) displayed high specificity against the SOX2 protein of human, mouse and rat.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Rabbit Anti-SOX2 Antibody (ZG-0015U) in WB
Positive WB detected in: U-251 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: SOX2 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa
Figure 2 Rabbit Anti-SOX2 Antibody (ZG-0015U) in IHC
IHC image of ZG-0015U diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human SOX2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SOX2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2E1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Microphthalmia, Syndromic 3 and Septooptic Dysplasia
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SOX2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2438z | Mouse Anti-SOX2 Recombinant Antibody (clone 44A5) | WB, ELISA, IHC | Mouse IgG2b, κ |
ZG-108R | Mouse Anti-SOX2 Recombinant Antibody (ZG-108R) | WB | Mouse IgG |
ZG-109R | Mouse Anti-SOX2 Recombinant Antibody (ZG-109R) | WB, ELISA | Mouse IgG |
VS3-CJ83 | Mouse Anti-SOX2 Recombinant Antibody (VS3-CJ83) | WB, ELISA | Mouse IgG |
VS3-XY1456 | Mouse Anti-SOX2 Recombinant Antibody (clone 10F10) | ELISA, WB, IHC, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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