Mouse Anti-SP1 Recombinant Antibody (clone 1326CT463.109.176)

Name: Mouse Anti-SP1 Recombinant Antibody (clone 1326CT463.109.176)
SKU: VS3-QX1046
Rating: 5 (1 reviews)

CAT#: VS3-QX1046

This product is a mouse antibody that recognizes SP1. The antibody can be used in applications: WB, ICC, FC.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12292/if_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_6_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12292/95_H4_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_B_8_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_7_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_7_4.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_8_5.jpg

Normal Tissue Figure 9 Testis

Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_4_6.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_8_8.jpg

RNA Expression Figure 11 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185591-SP1

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Specifications

Immunogen
Recombination protein from the human SP1.

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG1

Specificity
SP1

Species Reactivity
Human

Clone
1326CT463.109.176

Applications
WB, ICC, FC

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1:1000; ICC: 1:25; FC: 1:25

Target

Alternative Names
Sp1 Transcription Factor; Specificity Protein 1; TSFP1

Gene ID
6667

UniProt ID
P08047

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Related Signaling Pathways

MAPK Signaling Pathway

TGF-β Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-QX1046.pdf

MSDS

MSDS - VS3-QX1046.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

See other products for "SP1"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
NAB-1233-VHH	Recombinant Anti-human SP1 VHH Single Domain Antibody	IHC, ICC, IP, ChiP, Neut	Llama VHH

CAT	Product Name	Application	Type
MOB-2514z	Mouse Anti-SP1 Recombinant Antibody (clone 22G3)	WB, IHC, ELISA	Mouse IgG1
MOB-1915CT	Recombinant Mouse anti-Human SP1 Monoclonal antibody (4C8)	ELISA, FC, ICC, IF, IHC-P, WB
VS3-CJ483	Mouse Anti-SP1 Recombinant Antibody (VS3-CJ483)	WB, IF, FC	Mouse IgG1, κ
VS3-FY1376	Recombinant Rabbit Anti-SP1 Antibody (clone R01-9G6)	WB, IHC-Fr, IHC-P, ICC, IF	Rabbit IgG
VS3-FY1377	Recombinant Rabbit Anti-SP1 Antibody (clone R06-9I9)	WB, IHC-Fr, IHC-P, ICC, IF, IP	Rabbit IgG

CAT	Product Name	Application	Type
MOR-3379	Hi-Affi™ Recombinant Rabbit Anti-SP1 Monoclonal Antibody (DS3379AB)	WB, IHC, ICC	IgG

CAT	Product Name	Application
VS-0325-XY2154	Anti-SP1 Immunohistochemistry Kit	IHC
VS-0525-XY6834	Anti-Mouse SP1 Immunohistochemistry Kit	IHC
VS-0525-XY6833	Anti-Human SP1 Immunohistochemistry Kit	IHC
VS-0525-XY6835	Anti-Bovine SP1 Immunohistochemistry Kit	IHC

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