CytoStream™ Rabbit Anti-SPHK1 Recombinant Antibody (VS13-YC1103)
CAT#: VS13-YC1103
The CytoStream™ Rabbit Anti-SPHK1 Recombinant Antibody is specific for human SPHK1. SPHK1 encodes an enzyme forming sphingosine-1-phosphate, a key TNF-alpha and NF-kappa-B pathway mediator, influencing inflammation and apoptosis with several splicing variants. The anti-SPHK1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22829/if_selected.jpg
Normal Tissue
Figure 2 Duodenum
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28761/60067_A_8_2.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28761/60067_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28761/60067_A_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Hep G2, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176170-SPHK1
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Specifications
- Immunogen
- Recombinant human SPHK1, aa 40-80.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPHK1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50
Target
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Related Signaling Pathways
FcεR1 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SPHK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3384 | Hi-Affi™ Recombinant Rabbit Anti-SPHK1 Monoclonal Antibody (DS3384AB) | IHC-P, WB, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1429-CN | Recombinant Rabbit Anti-SPHK1 Monoclonal Antibody (JA31-14) | WB, IHC, FC | Rabbit IgG |
| VS3-WK244 | Rabbit Anti-SPHK1 Recombinant Antibody (clone JA31-14) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6857 | Anti-SPHK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6858 | Anti-Rat SPHK1 Immunohistochemistry Kit | IHC |
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