Recombinant Rabbit Anti-SPI1 Monoclonal Antibody (JB72-35)
CAT#: MRO-1297-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to SPI1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44653/1676_D3_2_selected.jpg
Normal Tissue
Figure 2 Bone marrow
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44653/125448_B_6_4_val_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_A_9_3.jpg
Normal Tissue
Figure 4 Skin
Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_B_9_1.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066336-SPI1
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Specifications
- Immunogen
- Recombinant protein within human PU.1/Spi1 aa 1-100
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- JB72-35
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- Spi-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 KDa Transforming Protein; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene; 31 KDa-Transforming Protein; Transcription Factor PU.1;
- Gene ID
- 6688
- UniProt ID
- P17947
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone JB72-35"
- CAT
- Product Name
See other products for "SPI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4037z | Mouse Anti-SPI1 Recombinant Antibody (clone 24D1) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-1727CT | Recombinant Mouse anti-Human SPI1 Monoclonal antibody (2G1) | ELISA, WB | |
| VS3-WK326 | Rabbit Anti-SPI1 Recombinant Antibody (clone JB72-35) | WB, ELISA | Rabbit IgG |
| VS3-CJ730 | Mouse Anti-SPI1 Recombinant Antibody (VS3-CJ730) | WB | Mouse IgG1, κ |
| VS3-FY1861 | Rabbit Anti-SPI1 Recombinant Antibody (clone R07-4D3) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3386 | Hi-Affi™ Recombinant Rabbit Anti-SPI1 Monoclonal Antibody (DS3386AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1105 | CytoStream™ Rabbit Anti-SPI1 Recombinant Antibody (VS13-YC1105) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2162 | Anti-SPI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6860 | Anti-Human SPI1 Immunohistochemistry Kit | IHC |
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