Rabbit Anti-SPI1 Recombinant Antibody (clone R07-4D3) (CAT#: VS3-FY1861)

• Immunogen
• Synthetic peptide of human PU.1/Spi1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Mouse SPI1

• Species Reactivity
• Human, Mouse

• Clone
• R07-4D3

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• MW
• The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 42 kDa.

• Related Disease
• Agammaglobulinemia 10, Autosomal Dominant and Autosomal Agammaglobulinemia

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• Spi-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 KDa Transforming Protein; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene; 31 KDa-Transforming Protein; Transcription Factor PU.1

• Gene ID
• 6688

• UniProt ID
• P17947

• Sequence Similarities
• Belongs to the ETS family.

• Cellular Localization
• Nucleus

• Protein Refseq
• NP_001074016.1; NP_003111.2

• Function
• Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions.
  Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
  Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
  May be particularly important for the pro- to pre-B cell transition.
  Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box.
  in vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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