Mouse Anti-SQSTM1 Recombinant Antibody (clone 5H7E2)
CAT#: VS7-0425-WR917
This product is a mouse IgG1 antibody that binds to human SQSTM1. The antibody 5H7E2 recognizes specific amino acid domain of human SP17 (AA: 1-152). It can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64165/1718_H4_8_cr57f3f8bf9c23b_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68843/158940_A_1_3_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/151358_A_7_8.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/151358_A_6_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161011-SQSTM1
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Specifications
- Immunogen
- Recombinant human SQSTM1 (AA: 232-356) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SQSTM1
- Species Reactivity
- Human
- Clone
- 5H7E2
- Molecule Class
- Ubiquitin-Binding Protein
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Paget disease of bone 3 (PDB3); Frontotemporal dementia and/or amyotrophic lateral sclerosis 3 (FTDALS3); Myopathy, distal, with rimmed vacuoles (DMRV)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Sequestosome 1; Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa; EBI3-Associated Protein Of 60 KDa; Oxidative Stress Induced Like; Ubiquitin-Binding Protein P62; EBIAP; OSIL; P60; Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62; EBI3-Associated Protein P60; Paget Disease Of Bone 3
- Gene ID
- 8878
- UniProt ID
- Q13501
- Long Name
- Sequestosome 1
- Cellular Localization
- Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Endosome; Lysosome; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Lipoprotein; Palmitate; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001135770.1
- Function
- Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes;; Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps;; SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies;; SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins;; SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed:16286508, PubMed:17580304, PubMed:37802024).; Also required to recruit ubiquitinated proteins to PML bodies in the nucleus;; Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes;; Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective gene;; Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes;; Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transportp;; Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation.; May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1;; May play a role in titin/TTN downstream signaling in muscle cells.; Adapter that mediates the interaction between TRAF6 and CYLD (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
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