Mouse Anti-SQSTM1 Recombinant Antibody (clone 5H7E2) (CAT#: VS7-0425-WR917)

• Immunogen
• Recombinant human SQSTM1 (AA: 232-356) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human SQSTM1

• Species Reactivity
• Human

• Clone
• 5H7E2

• Molecule Class
• Ubiquitin-Binding Protein

• Applications
• WB, IHC, FC

• Conjugate
• Unconjugated

• Related Disease
• Paget disease of bone 3 (PDB3); Frontotemporal dementia and/or amyotrophic lateral sclerosis 3 (FTDALS3); Myopathy, distal, with rimmed vacuoles (DMRV)

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• Sequestosome 1; Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa; EBI3-Associated Protein Of 60 KDa; Oxidative Stress Induced Like; Ubiquitin-Binding Protein P62; EBIAP; OSIL; P60; Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62; EBI3-Associated Protein P60; Paget Disease Of Bone 3

• Gene ID
• 8878

• UniProt ID
• Q13501

• Long Name
• Sequestosome 1

• Cellular Localization
• Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Endosome; Lysosome; Nucleus

• Post Translation Modifications
• Acetylation; Isopeptide bond; Lipoprotein; Palmitate; Phosphoprotein; Ubl conjugation

• Protein Refseq
• NP_001135770.1

• Function
• Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes;; Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps;; SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies;; SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins;; SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed:16286508, PubMed:17580304, PubMed:37802024).; Also required to recruit ubiquitinated proteins to PML bodies in the nucleus;; Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes;; Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective gene;; Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes;; Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transportp;; Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation.; May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1;; May play a role in titin/TTN downstream signaling in muscle cells.; Adapter that mediates the interaction between TRAF6 and CYLD (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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