CytoStream™ Mouse Anti-SQSTM1 Recombinant Antibody (VS13-YC1109) (CAT#: VS13-YC1109)
The CytoStream™ Mouse Anti-SQSTM1 Recombinant Antibody is specific for human, mouse and rat SQSTM1. SQSTM1 encodes a multifunctional protein regulating NF-kB signaling, acting with TRAF6, linked to bone diseases like Paget's with alternate splicing. The anti-SQSTM1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64165/1718_H4_8_cr57f3f8bf9c23b_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64165/1718_H4_8_cr57f3f8bf9c23b_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/if_selected.jpg
Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68843/158940_A_1_3_selected.jpg
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68843/158940_A_1_3_selected.jpg
Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/ihc_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/151358_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/151358_A_7_8.jpg
Figure 6 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/151358_A_6_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4587/151358_A_6_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161011-SQSTM1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161011-SQSTM1
Specifications
- Immunogen
- Recombinant human Sequestosome-1, aa 42-91.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat SQSTM1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Sporadic and familial Paget disease of bone
Product Property
- Clonality
- Monoclonal
- Purification
- Immunogen affinity purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/200
Target
- Alternative Names
- Sequestosome 1; Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa; EBI3-Associated Protein Of 60 KDa; Oxidative Stress Induced Like; Ubiquitin-Binding Protein P62; EBIAP; OSIL; P60; Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62; EBI3-Associated Protein P60; Paget Disease Of Bone 3;
- Gene ID
- 8878
- UniProt ID
- Q13501
- Long Name
- Sequestosome 1
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_001135770.1; NP_001135771.1; NP_003891.1
- Function
- Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a a bridge between polyubiquitinated proteins and autophagosomes.
Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2420CT | Recombinant Mouse anti-Human SQSTM1 Monoclonal antibody (EML854) | IHC-P, WB, ICC/IF | |
| MRO-1378-CN | Recombinant Mouse Anti-SQSTM1 Monoclonal Antibody (10-E10) | WB, IF, IHC, FC | Mouse IgG1 |
| MRO-2198-CN | Rabbit Anti-SQSTM1 Polyclonal Antibody (MRO-2198-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX1050 | Mouse Anti-SQSTM1 Recombinant Antibody (clone 3F8-E6-G9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX1051 | Mouse Anti-SQSTM1 Recombinant Antibody (clone 1336CT763.152.125) | WB | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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