CytoStream™ Rabbit Anti-SRC Recombinant Antibody (VS13-YC1110) (CAT#: VS13-YC1110)
The CytoStream™ Rabbit Anti-SRC Recombinant Antibody is specific for human, mouse and rat SRC. SRC is a proto-oncogene encoding a tyrosine-protein kinase, regulating development and growth; mutations relate to colon cancer. The anti-SRC antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/850_G12_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/850_G12_2_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows membranous and cytoplasmic positivity in cells in seminferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows membranous and cytoplasmic positivity in cells in seminferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_B_9_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_B_9_5.jpg
Figure 4 Stomach
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_1.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_1.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_8_3.jpg
Figure 6 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (A549, HBEC3-KT, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197122-SRC
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197122-SRC
Specifications
- Immunogen
- Recombinant human Src, aa 20-60.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SRC
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/5000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase; V-Src Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog; Proto-Oncogene C-Src; EC 2.7.10.2; P60-Src; SRC1; Proto-Oncogene Tyrosine-Protein Kinase Src; Protooncogene SRC, Rous Sarcoma;
- Gene ID
- 6714
- UniProt ID
- P12931
- Long Name
- SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
- Cellular Localization
- Nucleus; Cytoplasm; Cell membrane; Mitochondrion inner membrane
- Protein Refseq
- NP_005408.1; NP_938033.1
- Function
- Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1 (PubMed:11389730).
Plays a role in EGF-mediated calcium-activated chloride channel activation (PubMed:18586953).
Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'.
Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Bladder Cancer
Throid Cancer
Endocrine Resistance
cAMP Signaling Pathway
ErbB Signaling Pathway
MAPK Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1800MZ | Recombinant Mouse Anti-Chicken SRC Antibody (clone 0.U.249) | ICC, WB | Mouse antibody |
| MRO-1430-CN | Recombinant Rabbit Anti-SRC Monoclonal Antibody (CBACN-525) | WB, IF, IHC | Rabbit IgG |
| MRO-1431-CN | Recombinant Rabbit Anti-SRC Monoclonal Antibody (JF0947) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX1052 | Mouse Anti-SRC Recombinant Antibody (clone 17AT28) | WB, ICC | Mouse IgG1 |
| VS3-QX1053 | Mouse Anti-SRC Recombinant Antibody (clone 1F11) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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