Mouse Anti-SSB Recombinant Antibody (clone 2) (CAT#: FAMAB-0193YC)

Provided is a mouse monoclonal antibody. The antibody is specific for human recombinant La antigen in ELISA and inhibition by soluble La. It also binds ssDNA and dsDNA.


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ELISA

Figure 1 Inhibition of anti-La binding by soluble La protein.

Figure 1 Inhibition of anti-La binding by soluble La protein.

Purified IgG anti-La monoclonal antibodies were pre-incubated with various concentrations of purified recombinant La protein and tested by ELISA for binding to polystyrene plates coated with 1μg/ml of La protein.

Bloom, D. D., St Clair, E. W., Pisetsky, D. S., & Clarke, S. H. (1994). The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. European journal of immunology, 24(6), 1332–1338.

IP

Figure 2 Immunoprecipitation of <sup>32</sup>P-labeled HeLa cell extracts.

Figure 2 Immunoprecipitation of 32P-labeled HeLa cell extracts.

Lane 1, lpr #2; lane 2, Ipr #5; lane 3, Ipr#14; lane 4, lpr #15; lane 5, lpr #18; lane 6, lpr #19; lane 7, lpr#21; lane 8, IgG2a control antibody; lane 9, human anti-Laserum.

Bloom, D. D., St Clair, E. W., Pisetsky, D. S., & Clarke, S. H. (1994). The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. European journal of immunology, 24(6), 1332–1338.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Human SSB
  • Species Reactivity
  • Human
  • Clone
  • 2
  • Applications
  • ELISA, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • La; LARP3; La/SSB

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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