Rabbit Anti-STAG2 Recombinant Antibody (clone 3H4) (CAT#: ZG-0579U)
Rabbit Anti-STAG2 Antibody (clone 3H4) displayed high specificity against the STAG2 protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-STAG2 Antibody (ZG-0579U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: SA2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 142, 146 kDa
Observed band size: 142 kDa
IHC
Figure 2 Rabbit Anti-STAG2 Antibody (ZG-0579U) in IHC
Immunofluorescence staining of MCF7 Cells with ZG-0579U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human SA2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human STAG2
- Species Reactivity
- Human
- Clone
- 3H4
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Mullegama-Klein-Martinez Syndrome and Holoprosencephaly 13, X-Linked.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Cohesin subunit SA-2; SCC3 homolog 2; Stromal antigen 2; STAG2; SA2
- Gene ID
- 10735
- UniProt ID
- Q8N3U4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| MOB-2918z | Mouse Anti-STAG2 Recombinant Antibody (clone 19H7) | WB, ELISA, IF, IHC | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
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