Anti-STAT3 Antibody Prodrug, Protease Activated (VS-1025-YC60)
CAT#: VS-1025-YC60
The Anti-STAT3 Antibody Prodrug, Protease Activated (VS-1025-YC60) is an antibody prodrug comprised of a recombinant human antibody targeting human STAT3, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to STAT3 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to STAT3 effectively. STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor that is constitutively active in a variety of tumors, acting as a crucial mediator of oncogenic signaling from many RTKs (like EGFR and HER2). Its activation leads to the transcription of genes that promote cell survival, proliferation, and immune suppression. Inhibiting the active STAT3 protein is a goal in developing new anti-cancer drugs.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1671/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear positivity and cytoplasmic in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1671/155480_A_3_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58603/ihc_selected.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68241/153085_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_8_8.jpg
Normal Tissue
Figure 7 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity: 75%-25% Alveolar cells type I Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Macrophages Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_3_4.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_7_3.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168610-STAT3
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human STAT3
- Target
- STAT3
- Species Reactivity
- Human
- Isotype
- IgG
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
- Alternative Names
- APRF; HIES; ADMIO; ADMIO1
- Gene ID
- 6774
- UniProt ID
- P40763
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Related Diseases
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Throid Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
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| MOB-1395z-F(E) | Recombinant Anti-Human STAT3 Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MOB-1395z-S(P) | Recombinant Anti-Human STAT3 Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| MOB-1954CT | Recombinant Mouse anti-Human STAT3 Monoclonal antibody (EML1948) | WB |
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| BRD-0565MZ | Chicken Anti-STAT3 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MOR-4711 | Hi-Affi™ Recombinant Rabbit Anti-STAT3 Monoclonal Antibody (TH225DS) | WB, ELISA | IgG |
| CAT | Product Name | Application | Type |
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| MOB-113CQ | Llama Anti-STAT3 VHH Single Domain Antibody | WB, IP, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
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| MOB-092LC | Recombinant Llama Anti-STAT3 VHH Single Domain Antibody | ELISA, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
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| MRO-1438-CN | Recombinant Rabbit Anti-STAT3 Monoclonal Antibody (CBACN-529) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1439-CN | Recombinant Rabbit Anti-STAT3 Monoclonal Antibody (CBACN-530) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2385-CN | Recombinant Rabbit Anti-STAT3 (phosphorylated Ser727) Monoclonal Antibody (CBACN-670) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2386-CN | Recombinant Rabbit Anti-STAT3 (phosphorylated Thryr705) Monoclonal Antibody (CBACN-671) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0660-YC-S(P) | Human Anti-STAT3 Recombinant Antibody; scFv Fragment (HPAB-M0660-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0661-YC-S(P) | Human Anti-STAT3 Recombinant Antibody; scFv Fragment (HPAB-M0661-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0660-YC-F(E) | Human Anti-STAT3 Recombinant Antibody; Fab Fragment (HPAB-M0660-YC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0661-YC-F(E) | Human Anti-STAT3 Recombinant Antibody; Fab Fragment (HPAB-M0661-YC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP506-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP506-YC) | ELISA | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP507-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP507-YC) | ELISA | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP508-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP508-YC) | ELISA | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0051-FY | Rabbit Anti-STAT3 Recombinant Antibody (clone AFY0022) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0053-FY | Rabbit Anti-STAT3 Recombinant Antibody (clone AFY0024) | IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY249 | AbPlus™ Anti-STAT3 Magnetic Beads (CBACN-530) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY28 | Human Anti-STAT3 scFv-Fc Chimera (VS-0425-FY28) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2195 | Anti-STAT3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6951 | Anti-Mouse STAT3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6950 | Anti-Human STAT3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6953 | Anti-Zebrafish STAT3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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