Mouse Anti-STAT3 Recombinant Antibody (ZG-115R)
CAT#: ZG-115R
This product is a mouse antibody that recognizes STAT3. It can be used in a wide variety of applications, such as: WB, ELISA, IHC, IF.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-STAT3 Antibody (ZG-115R) in WB
Western Blot analysis using Stat3 monoclonal antibody against HeLa(1), NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS7 (5) cell lysate.
IHC
Figure 2 Mouse Anti-STAT3 Antibody (ZG-115R) in IHC
Immunohistochemistry analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) with DAB staining using Stat3 monoclonal antibody.
IF
Figure 3 Mouse Anti-STAT3 Antibody (ZG-115R) in IF
Immunofluorescence analysis of Hela cells using Stat3 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1671/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear positivity and cytoplasmic in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1671/155480_A_3_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58603/ihc_selected.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68241/153085_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_8_8.jpg
Normal Tissue
Figure 7 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity: 75%-25% Alveolar cells type I Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Macrophages Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_3_4.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_7_3.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168610-STAT3
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Specifications
- Immunogen
- Purified recombinant fragment of human Stat3 expressed in E. coli
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human, Mouse, Monkey
- Applications
- WB, ELISA, IHC, IF
Product Property
- Purification
- Affinity purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:2000, IHC: 1:200-1:1000, IF: 1:200-1:1000, ELISA: 1:10000
Target
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Related Diseases
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Throid Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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