Rabbit Anti-SUMO2/SUMO3 Recombinant Antibody (VS3-CJ1049)

CAT#: VS3-CJ1049

This product is a rabbit antibody that recognizes human, mouse, and rat SUMO2/SUMO3.

Specifications

  • Immunogen
  • Recombinant protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat SUMO2/SUMO3
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, ICC, IF, IHC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 40% Glycerol, 1% BSA, TBS, pH7.4.
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.

Target

  • Alternative Names
  • HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2; SMT3A; Smt3B; SMT3H1; SUMO-3
  • Sequence Similarities
  • Belongs to the ubiquitin family. SUMO subfamily.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Polymeric chains can be formed through Lys-11 cross-linking.
    Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
  • Function
  • Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678).
    Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

See other products for "SUMO2/SUMO3"

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CAT Product Name Application Type
MRO-1451-CN Recombinant Rabbit Anti-SUMO2/SUMO3 Monoclonal Antibody (CBACN-537) WB, IF, IHC Rabbit IgG
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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