Rabbit Anti-TAF3 Recombinant Antibody (clone R01-6A4) (CAT#: VS3-FY1602)
The rabbit recombinant monoclonal antibody has been tested in western blot analysis of TAF3 in Jurkat lysates. It has been shown to be effective to target human TAF3. It is suitable for use in a variety of assays, including WB, IHC-P. The R01-6A4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
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WB
Figure 1 Anti-TAF3 Antibody (VS3-FY1602) in WB.
The TAF3 expression in Jurkat lysates were detected using the anti-TAF3 by western blot assay.
IHC
Figure 2 Anti-TAF3 Antibody (VS3-FY1602) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-TAF3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Recombinant human TAF3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TAF3
- Species Reactivity
- Human
- Clone
- R01-6A4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 104 kDa, while the observed molecular weight stands at 140 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- TAF140; TAFII140; TAFII-140
- Gene ID
- 83860
- UniProt ID
- Q5VWG9
- Sequence Similarities
- Belongs to the TAF3 family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation
- Protein Refseq
- NP_114129.1
- Function
- The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription.
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13.
The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C.
TAF3 forms the TFIID-A module together with TAF5 and TBP.
Required in complex with TBPL2 for the differentiation of myoblasts into myocytes.
The TAF3-TBPL2 complex replaces TFIID at specific promoters at an early stage in the differentiation process.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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