Human Anti-TCDB Recombinant Antibody (clone ViF087_A10) (CAT#: VS-0623-WK25)

The antibody clone ViF087_A10 was produced by immunizing with full length TcdB from Clostridioides difficile. It can be used for detection of Clostridioides difficile TcdB in ELISA and WB assays.

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  • Published Data
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  • COA
Neut

Figure 1 IC<sub>50</sub> of ViF087_A10 and SH1429_B1 were estimated with serial antibody dilutions.

Figure 1 IC50 of ViF087_A10 and SH1429_B1 were estimated with serial antibody dilutions.

TcdBFL neutralization of ViF087_A10 and SH1429_B1 was further verified using serial dilutions of these antibodies

ELISA

Figure 2 Antigen ELISA on TcdB variants of antibodies including ViF087_A10.

Figure 2 Antigen ELISA on TcdB variants of antibodies including ViF087_A10.

The one most potent neutralizing antibodies (ViF087_A10) bind to epitopes within TcdB1−1852.

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Figure 3 <em>In vitro</em> neutralization of TcdB including antibody ViF087_A10.

Figure 3 In vitro neutralization of TcdB including antibody ViF087_A10.

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Figure 4 <em>In vitro</em> neutralization of TcdB with antibody combinations. TcdB (0.1 pM) and 1 nM of ViF087_A10 mAbs were coincubated in cultivation media with 100 nM of CROPs binding mAbs.

Figure 4 In vitro neutralization of TcdB with antibody combinations. TcdB (0.1 pM) and 1 nM of ViF087_A10 mAbs were coincubated in cultivation media with 100 nM of CROPs binding mAbs.


Specifications

  • Immunogen
  • Full length TcdB from Clostridioides difficile
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Clostridioides difficile TcdB1-1852
  • Species Reactivity
  • Clostridioides difficile
  • Clone
  • ViF087_A10
  • Conjugate
  • Unconjugated
  • Related Disease
  • CDI

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No Preservative
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Neutralization: 0.1 nM-100 nM

Target

  • Alternative Names
  • toxB
  • Long Name
  • Glycosylating toxin TcdB

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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