Rat Anti-TCR Recombinant Antibody (clone KJ16) (CAT#: FAMAB-0109-CN)

Figure 1 Immunoprecipitation with KJ16-133:

Normal T cells compared to DO-11.10. Normal T cells from BALB/c and SJA lymph node spleen were prepared.

Marrack, P. , & Kappler, J. . (1986). The antigen-specific, major histocompatibility complex-restricted receptor on t cells. Advances in Immunology, 38, 1.

Figure 2 Reactivity of KJ16-133 with T Cell Hybridomas and Tumors.

ELISA readings were calculated by averaging triplicate wells, subtracting background readings from wells incubated with cells and alkaline phosphatasecoupled rabbit anti-rat immunoglobulin but no primary antibody, and combining the errors appropriately to obtain standard errors for each corrected reading.

Marrack, P. , & Kappler, J. . (1986). The antigen-specific, major histocompatibility complex-restricted receptor on t cells. Advances in Immunology, 38, 1.

Figure 3 KJ16-133 stains only a portion of peripheral T cells in BALB/c mice.

In some cases these cells had been preincubated at 4°C or 37°C in azide with saturating amounts of KJ 16-133. Cells were then thoroughly washed and analyzed for fluorescence staining using a cytofluorograph.

Marrack, P. , & Kappler, J. . (1986). The antigen-specific, major histocompatibility complex-restricted receptor on t cells. Advances in Immunology, 38, 1.