Rabbit Anti-TEC Recombinant Antibody (clone R09-6E7)
CAT#: VS3-FY1899
This anti-Tec antibody detected positive in western blot analysis of Tec in K562 lysates. The recombinant monoclonal antibody has been meticulously designed to target human TEC. It is suitable for use in a variety of assays, including WB, ICC/IF. The R09-6E7 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200).
Tested Data
Gene Expression
WB
Figure 1 Anti-TEC Antibody (VS3-FY1899) in WB.
The Tec expression in K562 lysates were detected using the anti-Tec by western blot assay.
ICC
Figure 2 Anti-TEC Antibody (VS3-FY1899) in ICC.
Immunocytochemistry staining of Tec (green) in Hela using the anti-Tec antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24657/1846_C1_18_cr5ab92a9e55b74_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5733/20252_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5733/20252_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5733/20252_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5733/20252_A_8_8.jpg
Normal Tissue
Figure 6 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5733/20252_A_5_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135605-TEC
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Specifications
- Immunogen
- Synthetic peptide of human Tec
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TEC
- Species Reactivity
- Human
- Clone
- R09-6E7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 74 kDa, while the observed molecular weight stands at 58-75 kDa.
- Related Disease
- Transient Erythroblastopenia Of Childhood and Agammaglobulinemia, X-Linked
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Tec Protein Tyrosine Kinase; EC 2.7.10.2; PSCTK4; Tyrosine-Protein Kinase Tec; EC 2.7.10
- Gene ID
- 7006
- UniProt ID
- P42680
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Membrane
- Post Translation Modifications
- Following B-cell or T-cell receptors engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-519.
Undergoes also tyrosine phosphorylation during platelet activation.
(P42680-TEC_HUMAN)
Modification sites at PhosphoSitePlus (P42680)
Modification sites at neXtProt (NX_P42680)
- Protein Refseq
- XP_011512039.1; XP_011512043.2; XP_047272062.1; XP_047272063.1; XP_047272064.1
- Function
- Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TEC"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3529 | Rabbit Anti-TEC Recombinant Antibody (clone DS3529AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-133R | Mouse Anti-TEC Recombinant Antibody (ZG-133R) | WB, ELISA | Mouse IgG |
| VS3-XY1493 | Mouse Anti-TEC Recombinant Antibody (clone 3A5) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7166 | Anti-TEC Immunohistochemistry Kit | IHC |
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