Human Anti-Testosterone Recombinant Antibody (clone TM-4) (CAT#: FAMAB-0302-CN)
This is a human monoclonal antibody which specifically reacts with Testosterone. The antibody TM-4 binds to Testosterone with high affinity, which shows a KD of 2 nM.
Specific Inquiry
ELISA
Figure 1 Dose-dependent binding of scFv TM-4 to testosterone (squares) and testosterone-biotin (circles) as determined by competitive ELISA.
The mean binding signal, average of duplicates, at each antigen concentration was divided by the antibody binding response obtained in the absence ofinhibiting antigen to give percentage binding. The IC₅₀ values, being equal to the antigen concentration at which the binding response is half-maximum (50%), are 2 and 4 nM for testosterone and testosterone-biotin, respectively, thus making this antibody fragment suitable for measuring testosterone within the clinically relevant range (0.7–70 nM).
Persson, H., Wallmark, H., Ljungars, A., Hallborn, J., & Ohlin, M. (2008). In vitro evolution of an antibody fragment population to find high-affinity hapten binders. Protein Engineering, Design & Selection, 21(8), 485-493.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Testosterone
- Clone
- TM-4
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
- Alternative Names
- Testosterone
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone TM-4"
See other products for "Testosterone"
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-963-S(P) | Recombinant Anti-Testosterone Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| MHH-963-S(P) | Recombinant Human Anti-Testosterone Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| PSBC-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C(4)F(5)); scFv Fragment | WB, FuncS | Mouse scFv |
| PSBZ-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5); scFv Fragment | Block | Mouse scFv |
| PSBC-121 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5 mutant (77)); scFv Fragment | WB, ELISA | Mouse scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-100 | Recombinant Anti-human Testosterone VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C(4)F(5)) | WB, FuncS | Mouse IgG |
| PABZ-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5) | Block | Mouse IgG |
| PABC-121 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5 mutant (77)) | WB, ELISA | Mouse IgG |
| FAMAB-0283YC | Human Anti-Testosterone Recombinant Antibody (clone TC-5) | ELISA | Human IgG |
| FAMAB-0284YC | Human Anti-Testosterone Recombinant Antibody (clone TC-20) | ELISA | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C(4)F(5)); Fab Fragment | WB, FuncS | Mouse Fab |
| PFBZ-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5); Fab Fragment | Block | Mouse Fab |
| PFBC-121 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5 mutant (77)); Fab Fragment | WB, ELISA | Mouse Fab |
| FAMAB-0301-CN-F(E) | Human Anti-Testosterone Recombinant Antibody (clone TM-7); Fab Fragment | ELISA | Human Fab |
| FAMAB-0302-CN-F(E) | Human Anti-Testosterone Recombinant Antibody (clone TM-4); Fab Fragment | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-176 | Recombinant Mouse Anti-Testosterone Antibody (77Fab) | Neut, FuncS | IgG |
| PABX-176-S (P) | Recombinant Mouse Anti-Testosterone Antibody scFv Fragment (77Fab) | Neut, FuncS | scFv |
| PABX-176-F (E) | Recombinant Mouse Anti-Testosterone Antibody Fab Fragment (77Fab) | Neut, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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