Rat Anti-TET1 Recombinant Antibody (clone 0725W53)
CAT#: VS-0725-FY220
This recombinant antibody, clone 0725W53, targets a recombinant protein corresponding to amino acids 1682-1914 of mouse Tet1. It is validated for Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). TET1 is a demethylase belonging to the TET family, playing a crucial role in DNA methylation and gene activation. This antibody is a valuable tool for studying epigenetic mechanisms and gene expression control.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19032/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57273/if_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (BEWO, HAP1, K-562, NTERA-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000138336-TET1
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Specifications
- Immunogen
- A recombinant protein corresponding to amino acids 1682-1914 of mouse Tet1.
- Host Species
- Rat
- Type
- Rat IgG2a
- Specificity
- Human TET1
- Species Reactivity
- Human, Mouse
- Clone
- 0725W53
- Applications
- Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
- Related Disease
- Acute Myeloid Leukemia (AML)
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 215 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 4 µL per Chromatin Immunoprecipitation
Target
- Alternative Names
- Tet Methylcytosine Dioxygenase 1; Leukemia-Associated Protein With A CXXC Domain; Ten-Eleven Translocation 1 Gene Protein; CXXC-Type Zinc Finger Protein 6; Ten-Eleven Translocation-1; CXXC Zinc Finger 6; Tet Oncogene 1; EC 1.14.11.n2; CXXC6
- Gene ID
- 80312
- UniProt ID
- Q8NFU7
- Long Name
- Tet Methylcytosine Dioxygenase 1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TET1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3979z | Mouse Anti-TET1 Recombinant Antibody (clone 20G10) | WB, IP, ELISA | Mouse IgG2a, κ |
| MRO-2217-CN | Rabbit Anti-TET1 Polyclonal Antibody (MRO-2217-CN) | IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7176 | Anti-TET1 Immunohistochemistry Kit | IHC |
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