Mouse Anti-TET2 Recombinant Antibody (VS3-FY2890)
CAT#: VS3-FY2890
This anti-TET2 antibody is a mouse recombinant monoclonal antibody that has been meticulously designed to target the human TET2 protein, a calculated molecular weight of the protein is about 223.8 kDa. The antibody can be utilized for detection of TET2 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200) and Immunocytochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_A_4_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_B_6_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43135/95044_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168769-TET2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TET2
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 223.8 kDa.
- Related Disease
- Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation.
Has a preference for 5-hydroxymethylcytosine in CpG motifs.
Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC).
Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation.
Methylation at the C5 position o
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Tet Methylcytosine Dioxygenase 2; KIAA1546; Tet Oncogene Family Member 2; EC 1.14.11.n2; Probable Methylcytosine Dioxygenase TET2; Methylcytosine Dioxygenase TET2; MDS
- Gene ID
- 54790
- UniProt ID
- Q6N021
- Sequence Similarities
- Belongs to the TET family.
- Cellular Localization
- Nucleus
Chromosome
- Post Translation Modifications
- May be glycosylated.
Monoubiquitinated at Lys-1299 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex; this modification promotes binding to DNA. (Q6N021-TET2_HUMAN)
Modification sites at PhosphoSitePlus (Q6N021)
Modification sites at neXtProt (NX_Q6N021)
Glycosylation from GlyGen (Q6N021) 9 sites, 1 O-linked glycan (9 sites)
- Protein Refseq
- XP_006714305.1; XP_016863808.1; XP_024309870.1; XP_024309871.1
- Function
- Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation.
Has a preference for 5-hydroxymethylcytosine in CpG motifs.
Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC).
Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation.
Methylation at the C5 position o
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TET2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3594z | Mouse Anti-TET2 Recombinant Antibody (clone 31B4) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
| VS7-0425-WR964 | Mouse Anti-TET2 Recombinant Antibody (clone 5H8B3) | FC | Mouse IgG1 |
| VS7-0425-WR965 | Mouse Anti-TET2 Recombinant Antibody (clone 5H8C7) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC94 | Mouse Anti-TET2 Recombinant Antibody (VS-0924-YC94) - Cancer Stem Cell Marker | IHC, WB, IF | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7177 | Anti-TET2 Immunohistochemistry Kit | IHC |
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