Human Anti-Tetanus toxoid Recombinant Antibody (clone F13) (CAT#: VS-0623-WK14)
The antibody clone F13 was produced by immunizing with purified tetanus toxoid. It can be used for detection of C. tetani tetanus toxoid in ELISA and WB assays.
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WB
Figure 1 The reactivity of the Fab fragment with the tetanus toxoid in immuno blot analysis.
The purified F13 was electrophoresed on a 12% SDS-PAGE. The resolved proteins were transferred to a polyvinyl difluoride membrane following the manufacturer's instructions. The residual sites were blocked using 2% skimmed milk powder and probed with anti-human IgG (Fab-specific) HRPO 1:5000 (Sigma, St. Louis, USA). The blot was developed using 0.05% 3,3-diaminobenzidine tetrahydrochloride (DAB) (Sigma, USA) and 0.03% hydrogen peroxide in PBS.
ELISA
Figure 2 Analysis of antigen-binding affinity of F13 Fab by Indirect ELISA to evaluate the activity of Fab toward tetanus toxoid.
The F13 Fab showed signifi cant binding activity at high concentration (2 μg/100 μl) with the OD of ~1.0 and gradually reduced to ~0.1 with the decrease in concentration.
ELISA
Figure 3 Analysis of F13 Fab activity toward tetanus toxoid in sandwich ELISA.
2F8 Mouse mAb was coated as a capture antibody, and titration of tetanus toxoid antigen against a constant dilution of F13 Fab revealed a concentration-dependent reduction of the binding signal in the wells. The E. coli lysate used
as a negative control showed negligible binding activity with tetanus toxoid antigen in the ELISA plate.
ELISA
Figure 4 Competitive ELISA using the F13 Fab and the mouse monoclonal antibody.
Constant OD values (around ~1.0) in the dilution of both antibodies indicated that the F13 Fab and mouse mAb were binding to different antigenic sites on tetanus toxoid.
ELISA
Figure 5 Specificity of F13 Fab toward various Clostridium species toxins.
Neut
Figure 6 Neutralizing efficiency of F13 Fab toward tetanus toxoid by flocculation assay.
Neutralization expressed in the limit of flocculation.
Specifications
- Immunogen
- Purified tetanus toxoid
- Host Species
- Human
- Type
- Human IgG
- Specificity
- C. tetani tetanus toxoid
- Species Reactivity
- C. tetani
- Clone
- F13
- Applications
- ELISA, WB, Neut
- Conjugate
- Unconjugated
- Related Disease
- Tetanus
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Competitive ELISA: 1/500
Indirect ELISA: 20 μg/mL
Western blot: 200 μg/mL
Target
- Alternative Names
- Tetanus toxoid
- Long Name
- Tetanus toxoid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MHC Tetramer Products for Other Applications
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-965 | Recombinant Anti-C. tetani tetanus toxoid Antibody | ELISA, WB, FuncS | IgG |
| MHH-965 | Recombinant Human Anti-C. tetani tetanus toxoid Antibody | WB, IHC, FuncS | IgG |
| PABZ-121 | Human Anti-Tetanus Toxoid Recombinant Antibody (clone B7-15A2) | Neut | Human IgG |
| FAMAB-0065WJ | Human Anti-Tetanus toxoid Recombinant Antibody (clone F5-20) | Neut | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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