Rabbit Anti-TFEB Recombinant Antibody (VS3-CJ748) (CAT#: VS3-CJ748)
This product is a rabbit antibody that recognizes human, and mouse endogenous levels of total TFEB.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line HeLa shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67082/1645_F12_4_cr57b564e2472ae_selected.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112561-TFEB
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse endogenous levels of total TFEB
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 150mM NaCl, PBS, pH7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- TCFEB; BHLHE35; ALPHATFEB
- Gene ID
- 7942
- UniProt ID
- P19484
- Sequence Similarities
- Belongs to the MiT/TFE family.
- Cellular Localization
- Cytoplasm, Lysosome, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-211 by MTOR regulates its subcellular location and activity (PubMed:21617040, PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:24081491, PubMed:30120233, PubMed:35662396).
When nutrients are present, phosphorylation by MTOR promotes association with 14-3-3/YWHA adapters and retention in the cytosol (PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:35662396).
Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22576015, PubMed:22343943, PubMed:22692423).
Exported from the nucleus in a mTORC1-dependent manner in response to nutrient availability (PubMed:30120233).
Dephosphorylated by phosphatase PPP3CA following Coxsackievirus B3 infection, leading to nuclear translocation (PubMed:33691586).
Alkylated via a non-enzymatic covalent modification (PubMed:35662396).
Itaconate, an anti-inflammatory metabolite generated in response to lipopolysaccharide, alkylates Cys-212, preventing association with 14-3-3/YWHA adapters, thereby promoting nuclear translocation and activity (PubMed:35662396).
Sumoylated; does not affect dimerization with MITF.
(Microbial infection) Cleavage by Coxsackievirus B3 protease 3C after site Gln-60. This non-phosphorylated cleavage product retains its ability to interact with TFEB, TFE3 or MITF and presents impaired transcriptional activity, resulting in disruption of lysosomal functions and increased viral infection.
- Protein Refseq
- NP_001161299.2; NP_001258872.1; NP_001258873.1
- Function
- Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response (PubMed:21617040, PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:30120233, PubMed:31672913, PubMed:35662396).
Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF (PubMed:1748288, PubMed:19556463, PubMed:29146937).
Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation (PubMed:21617040, PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:35662396).
Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity (PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:35662396).
Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes (PubMed:19556463, PubMed:22692423).
Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).
Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy (PubMed:21617040, PubMed:22576015, PubMed:23434374, PubMed:27278822).
In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity (By similarity).
Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer (PubMed:2115126).
Plays a role in the signal transduction processes required for normal vascularization of the placenta (By similarity).
Involved in the immune response to infection by the bacteria S.aureus, S.typhimurium or S.enterica: infection promotes itaconate production, leading to alkylation, resulting in nuclear localization and transcription factor activity (PubMed:35662396).
Itaconate-mediated alkylation activates TFEB-dependent lysosomal biogenesis, facilitating the bacteria clearance during the antibacterial innate immune response (PubMed:35662396).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TFEB"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1543z | Mouse Anti-TFEB Recombinant Antibody (clone 30B6) | WB, ELISA, IF, IP | Mouse IgG1, κ |
MOB-1545MZ | Recombinant Mouse Anti-Human TFEB Antibody (clone 420D3b) | WB | Mouse antibody |
VS3-FY1439 | Recombinant Rabbit Anti-TFEB Antibody (clone R05-0D7) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
VS-0923-FY144 | Recombinant Mouse Anti-TFEB Antibody (VS-0923-FY144) | ELISA, WB | Mouse IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ748. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: ELISA
Application: ELISA, FC, Block, SPR
Application: WB, ELISA
Application: ELISA, FC, IF, IHC, WB
Application: ELISA, IHC, WB
Application: ELISA, FuncS
For Research Use Only. Not For Clinical Use.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.