Anti-TFRC (clone W24V47) Recombinant Antibody Coupled Liposome (VS-1024-FY50) (CAT#: VS-1024-FY50)

• Potential Clinical Applications
• Traversing the blood-brain barrier (BBB)

• Immunoliposomes Method
• The Tfr antibody were thiolated using a 40:1 molar excess of 2-iminothiolane at room temperature for one hour, following previously described protocols. The resulting thiolated Tfr antibody (OX26-SH) were then combined with PL/pEGFP and PL/pC27 in a weight ratio of peptide to phospholipid of 1:20, and incubated overnight at room temperature. Targeting liposome complexes were subsequently separated using a Sepharose CL-4B column, and eluted with 0.001 M PBS buffer (pH 7.4) from the reaction mixture. The concentration of uncoupled OX26 or Tfr antibody was measured using the NanoOrange Protein Quantitation Kit. The total and uncoupled amounts of Tfr antibody were denoted as C0 and C1, respectively. The coupling efficiency (CE) was calculated using the formula: CE = (1 - C1/C0) × 100%.

• Clone
• W24V47

• Antibody Type
• IgG2a

• Antibody Host
• Mouse

• Antibody Reactivity
• Rat

• Antibody Description
• The mouse anti-rat CD71 antibody, clone OX-26, specifically recognizes rat CD71, commonly referred to as the transferrin receptor. This receptor is a homodimeric type II transmembrane protein found on the surface of all proliferating cells and in cells that have a need for iron, such as reticulocytes and capillary endothelium within the brain. Additionally, clone OX-26 exhibits binding to various non-dividing normal tissues.

• Storage
• See in the COA

• Storage Shelf Time
• See in the COA

• Target
• TFRC

• Introduction
• This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.

• Alternative Names
• Transferrin Receptor; TFR1; TRFR; P90; TFR; TR; T9

• Full Name
• Transferrin Receptor

• Gene ID
• 7037

• UniProt ID
• P02786

• Cellular Localization
• Cell membrane, Membrane, Secreted

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