CytoStream™ Rat Anti-TFRC Recombinant Antibody (clone RI7217) (CAT#: VS-0225-XY206)
This rat-derived antibody specifically targets TFRC, a pivotal protein involved in iron transport. With its high reactivity to mouse TFRC, it is an essential tool for studying cellular iron regulation. Primarily optimized for flow cytometry, this antibody provides precise and reproducible results, making it an invaluable asset in research focused on cellular metabolism and immune function.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
Figure 5 Colon
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
Figure 6 Testis
(Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
(Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
Figure 7 Bone marrow
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
Figure 8 Lymph node
(Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
(Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000072274-TFRC
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000072274-TFRC
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
Specifications
- Immunogen
- Mouse erythroleukemia
- Host Species
- Rat
- Type
- Rat IgG2a, kappa
- Specificity
- TFRC
- Species Reactivity
- Mouse
- Clone
- RI7217
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS containing BSA
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G067E | Anti-TFRC immunotoxin HB21 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G067D | Anti-TFRC immunotoxin HB21 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G067G | Anti-TFRC immunotoxin HB21 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G067R | Anti-TFRC immunotoxin HB21 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G067S | Anti-TFRC immunotoxin HB21 (scFv)-Sap | Cytotoxicity assay, Function study |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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