Mouse Anti-TGFB1 Recombinant Antibody (clone 7F6)

Name: Mouse Anti-TGFB1 Recombinant Antibody (clone 7F6)
SKU: VS7-0425-WR978
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR978

This product is a mouse IgG1 antibody that binds to human TGFB1. The antibody 7F6 recognizes specific amino acid domain of human TGFb1 (AA: 62-195). It can be used for IHC and FC assays.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73356/1901_C17_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/361/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73356/1812_G3_5_cr5a09c41109818_blue_red_green.jpg

Normal Tissue Figure 4 Bone marrow

Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/361/2675_B_6_4.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105329-TGFB1

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Specifications

Immunogen
Recombinant human TGFB1 (AA: 62-195) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human TGFB1

Species Reactivity
Human

Clone
7F6

Molecule Class
Transforming Growth Factor

Applications
IHC, FC

Conjugate
Unconjugated

Related Disease
Camurati-Engelmann disease (CAEND); Inflammatory bowel disease, immunodeficiency, and encephalopathy (IBDIMDE)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
metelimumab;272780-74-2;CAT-192;TGFB1;transforming growth factor, beta 1;DPD1, TGFB;transforming growth factor beta-1;Camurati Engelmann disease;CED;TGFbeta;TGF-beta-1;TGF-beta 1 protein;latency-associated peptide;LAP;DPD1;TGFB;

Gene ID
7040

UniProt ID
P01137

Long Name
Transforming Growth Factor Beta 1

Cellular Localization
Extracellular matrix; Secreted

Post Translation Modifications
Cleavage on pair of basic residues; Disulfide bond; Glycoprotein

Protein Refseq
NP_000651.3

Function
Growth factor; Mitogen

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Related Diseases

Inflammatory Bowel Diseases

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR978.pdf

MSDS

MSDS - VS7-0425-WR978.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
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Secondary Antibody

CAT
Product Name

Reconstitution Buffer

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Product Name

Recommended Dilution Buffer

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