Mouse Anti-TGM2 Recombinant Antibody (clone 3H2B8)
CAT#: ZG-0188U
Mouse anti-TGM2 antibody (clone 3H2B8) displayed high specificity against the TGM2 protein of Human.
Tested Data
Gene Expression
IHC
Figure 2 Mouse Anti-TGM2 Antibody (ZG-0188U) in IHC
Immunohistochemical of paraffin-embedded Human placenta tissue using ZG-0188U at dilution of 1:200.
WB
Figure 1 Mouse Anti-TGM2 Antibody (ZG-0188U) in WB
All lanes: Mouse anti-TGM2 Monoclonal antibody at 1μg/ml
Lane 1 : A549 whole cell lysate
Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 77kd
Observed band size : 77,62,39KD
Additional bands at : 30kd
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29518/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2598/635_D8_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2598/639_D8_2_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29518/62634_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (CAPAN-2, EFO-21, hTERT-RPE1, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 5 RNA cell line category: Group enriched (CAPAN-2, EFO-21, hTERT-RPE1, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198959-TGM2
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Specifications
- Immunogen
- Recombinant Human Protein-glutamine gamma-glutamyltransferase 2 (TGM2)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TGM2
- Species Reactivity
- Human
- Clone
- 3H2B8
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Dermatitis Herpetiformis and Balanitis Xerotica Obliterans
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000,IHC:1:50-1:500
Target
- Alternative Names
- TGC; tTG; G(h); hTG2; TG(C); protein-glutamine gamma-glutamyltransferase 2; C polypeptide, protein-glutamine-gamma-glutamyltransferase; TGase C; TGase H; TGase II; TGase-2; erythrocyte transglutaminase; heart G alpha(h); hhG alpha(h); isopeptidase TGM2; protein G alpha(h); protein-glutamine deamidase TGM2; protein-glutamine dopaminyltransferase TGM2; protein-glutamine histaminyltransferase TGM2; protein-glutamine noradrenalinyltransferase TGM2; protein-glutamine serotonyltransferase TGM2; tTgase; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase II
- Gene ID
- 7052
- UniProt ID
- P21980
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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