Rabbit Anti-TH Recombinant Antibody (clone R01-3E4) (CAT#: VS3-FY1925)

• Immunogen
• Synthetic peptide of human tyrosine hydroxylase

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat TH

• Species Reactivity
• Human, Rat

• Clone
• R01-3E4

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence

• MW
• The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 59 kDa.

• Related Disease
• Segawa Syndrome, Autosomal Recessive and Dystonia

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200

• Alternative Names
• Tyrosine Hydroxylase; Tyrosine 3-Monooxygenase; Tyrosine 3-Hydroxylase; EC 1.14.16.2; TYH; Dystonia 14; EC 1.14.16; DYT14; DYT5b

• Gene ID
• 7054

• UniProt ID
• P07101

• Sequence Similarities
• Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

• Cellular Localization
• Cell projection, Cytoplasm, Cytoplasmic vesicle, Nucleus, Synapse

• Post Translation Modifications
• Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities.
  Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity (PubMed:7901013).
  Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines (PubMed:15287903).
  Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway ().
  Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network (PubMed:28637871).
  Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG; this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669).
  Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity ().

• Protein Refseq
• XP_011518637.1; XP_054225745.1; NP_000351.2; NP_954986.2; NP_954987.2

• Function
• Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody