Rabbit Anti-TH Recombinant Antibody (clone R01-3E4)
CAT#: VS3-FY1925
This R01-3E4 recombinant monoclonal antibody has been meticulously designed to target human and rat TH. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The binding specificity of the tyrosine 3 monooxygenase antibody was determined using western blot analysis of tyrosine hydroxylase in PC-12 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14010/1905_B8_3_selected.jpg
Normal Tissue
Figure 2 High expression in adrenal gland
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61003/159875_B_6_5_val_selected.jpg
Normal Tissue
Figure 3 Low expression in pancreas
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61003/160099_A_3_3_val_selected.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (PC-3, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000180176-TH
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WB
Figure 1 Anti-TH Antibody (VS3-FY1925) in WB.
The Tyrosine Hydroxylase expression in PC-12 lysates were detected using the anti-Tyrosine 3 Monooxygenase by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human tyrosine hydroxylase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat TH
- Species Reactivity
- Human, Rat
- Clone
- R01-3E4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 59 kDa.
- Related Disease
- Segawa Syndrome, Autosomal Recessive and Dystonia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Tyrosine Hydroxylase; Tyrosine 3-Monooxygenase; Tyrosine 3-Hydroxylase; EC 1.14.16.2; TYH; Dystonia 14; EC 1.14.16; DYT14; DYT5b
- Gene ID
- 7054
- UniProt ID
- P07101
- Sequence Similarities
- Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoplasmic vesicle, Nucleus, Synapse
- Post Translation Modifications
- Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities.
Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity (PubMed:7901013).
Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines (PubMed:15287903).
Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway ().
Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network (PubMed:28637871).
Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG; this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669).
Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity ().
- Protein Refseq
- XP_011518637.1; XP_054225745.1; NP_000351.2; NP_954986.2; NP_954987.2
- Function
- Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa.
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Related Diseases
Amphetamine Addiction
Alcoholism
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2042z | Mouse Anti-TH Recombinant Antibody (clone 26H11) | WB, ICC, IF | Mouse IgG2a |
| MRO-1545-CN | Rabbit Anti-TH Recombinant Antibody (clone CBACN-567) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1546-CN | Rabbit Anti-TH Recombinant Antibody (clone CBACN-568) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ456 | Rabbit Anti-TH Recombinant Antibody (VS3-CJ456) | WB, ICC, IHC, FC | Rabbit IgG |
| VS7-0425-WR979 | Mouse Anti-TH Recombinant Antibody (clone 1B8D2) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0734MZ | Chicken Anti-TH Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3558 | Rabbit Anti-TH Recombinant Antibody (clone DS3558AB) | WB, FC | Rabbit IgG |
| MOR-4763 | Rabbit Anti-TH Recombinant Antibody (clone TH277DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0099-FY | Rabbit Anti-TH Recombinant Antibody (clone AFY0070) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1269 | PE-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
| MHC-CN1270 | APC-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
| MHC-CN1271 | FITC-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
| MHC-CN1272 | H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC381 | AbPlus™ Anti-TH Magnetic Beads (VS-0724-YC381) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2280 | Anti-TH Immunohistochemistry Kit | IHC | |
| VS-0525-XY7225 | Anti-Mouse TH Immunohistochemistry Kit | IHC | |
| VS-0525-XY7224 | Anti-Human TH Immunohistochemistry Kit | IHC | |
| VS-0525-XY7226 | Anti-Rat TH Immunohistochemistry Kit | IHC |
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