Mouse Anti-TIM-1 Recombinant Antibody (clone 3B3) (CAT#: FAMAB-1499CQ)
This product is a recombinant mouse antibody that recognizes TIM-1. The antibody was purified by affinity chromatography. It can be used in Stim, WB, FC, IF, IHC.
Specific Inquiry
Figure 1 The 3B3 mAb is specific to TIM-1.
Untransfected 300.19 cells (300.19) or 300.19 cells stably transfected with mouse TIM-1 (300.19 mTIM-1) or mouse TIM-2 (300.19 mTIM-2) were stained with 3B3 mAb to TIM-1 (open histograms) or rat IgG isotype control (filled histograms).
Umetsu, S. E., Lee, W. L., McIntire, J. J., Downey, L., Sanjanwala, B., Akbari, O., ... & DeKruyff, R. H. (2005). TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nature immunology, 6(5), 447-454.
FC
Figure 2 TIM-1 expression on CD4+ T cells, CD8+ T cells, CD19+ B cells and CD11c+ bone marrow-derived DCs.
Spleen cells from BALB/c mice were purified with CD4+, CD19+ or CD8+ MACS columns, stained with FITC-conjugated mAbs to cell surface markers (above histograms), costained with phycoerythrin-conjugated 3B3 mAb to mouse TIM-1 or rat IgG2a isotype control (filled histograms) and analyzed by flow cytometry.
Umetsu, S. E., Lee, W. L., McIntire, J. J., Downey, L., Sanjanwala, B., Akbari, O., ... & DeKruyff, R. H. (2005). TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nature immunology, 6(5), 447-454.
FuncS
Figure 3 Costimulation of T cells by anti-TIM-1.
CD4+ T cells purified from TCR-transgenic DO11.10 mice were cultured with OVA and bone marrow-derived DCs in the presence of intact mAb 3B3 (10.0 ug/ml) or Fab fragments of mAb 3B3 (10 or 15 ug/ml).
Umetsu, S. E., Lee, W. L., McIntire, J. J., Downey, L., Sanjanwala, B., Akbari, O., ... & DeKruyff, R. H. (2005). TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nature immunology, 6(5), 447-454.
Specifications
- Immunogen
- Mmouse TIM-1-Ig
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse TIM-1
- Species Reactivity
- Mouse
- Clone
- 3B3
- Applications
- Stim, WB, FC, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- t cell immunoglobulin domain and mucin domain 1; Tim-1
- UniProt ID
- Q5QNS5
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Tim-1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-975 | Recombinant Anti-Tim-1 Antibody | WB, Dot, FuncS | IgG |
| MHH-975 | Recombinant Human Anti-Tim-1 Antibody | ELISA, WB, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-975-F(E) | Recombinant Anti-Tim-1 Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-975-F(E) | Recombinant Human Anti-Tim-1 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-975-S(P) | Recombinant Anti-Tim-1 Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
| MHH-975-S(P) | Recombinant Human Anti-Tim-1 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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