Mouse Anti-TIMD4 Recombinant Antibody (clone 2C6A3) (CAT#: VS7-0425-WR991)
This product is a mouse IgG1 antibody that binds to human TIMD4. The antibody 2C6A3 recognizes specific amino acid domain of human TIMD4 (AA: extra 25-314). It can be used for ICC and FC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/1873_G5_4_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/1873_G5_4_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows distinct positivity in macrophages.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows distinct positivity in macrophages.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26027/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26027/ihc_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/38243_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/38243_A_7_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/38243_A_8_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/38243_A_8_5.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/38243_A_5_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15625/38243_A_5_6.jpg
Figure 7 RNA cell line category: Group enriched (U-266/70, U-266/84)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145850-TIMD4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145850-TIMD4
Specifications
- Immunogen
- Recombinant human TIMD4 (AA: extra 25-314) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TIMD4
- Species Reactivity
- Human
- Clone
- 2C6A3
- Molecule Class
- Plasma Proteins
- Applications
- ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:50 - 1:200
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- T Cell Immunoglobulin And Mucin Domain Containing 4; T-Cell Immunoglobulin And Mucin Domain Containing 4; T-Cell Immunoglobulin Mucin Receptor 4; T-Cell Membrane Protein 4; TIMD-4; TIM-4; TIM4; T-Cell Immunoglobulin And Mucin Domain-Containing Protein 4; SMUCKLER
- Gene ID
- 91937
- UniProt ID
- Q96H15
- Long Name
- T Cell Immunoglobulin And Mucin Domain Containing 4
- Cellular Localization
- Cell membrane; Membrane; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001140198.1
- Function
- Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity).; Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed:32703939, PubMed:34067457).; Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (PubMed:32640697).; Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2028CQ | Rat Anti-Timd4 Recombinant Antibody (clone RMT4-53) | FC, ICC, IF, Neut | Rat IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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