Rabbit Anti-TIMELESS Recombinant Antibody (clone R01-7H7)
CAT#: VS3-FY1902
This R01-7H7 recombinant monoclonal antibody has been meticulously designed to target human TIMELESS. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The antibody has the positive detection of TIMELESS by western blot analysis of timeless in MCF-7, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60655/1103_H4_2_selected.jpg
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WB
Figure 1 Anti-TIMELESS Antibody (VS3-FY1902) in WB.
The Timeless expression in MCF-7, Hela lysates were detected using the anti-TIMELESS by western blot assay.
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Specifications
- Immunogen
- Human synthetic peptide Timeless
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TIMELESS
- Species Reactivity
- Human
- Clone
- R01-7H7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 139 kDa, while the observed molecular weight stands at 150 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Timeless Circadian Regulator; Timeless Circadian Clock 1; TIM1; HTIM; TIM; Timeless (Drosophila) Homolog; Timeless Homolog (Drosophila)
- Gene ID
- 8914
- UniProt ID
- Q9UNS1
- Sequence Similarities
- Belongs to the timeless family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys288 and Lys299 (NX_Q9UNS1 [NX_Q9UNS1-1])
Modification sites at PhosphoSitePlus (Q9UNS1)
- Protein Refseq
- NP_001317224.1; NP_003911.2
- Function
- Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TIMELESS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3572 | Rabbit Anti-TIMELESS Recombinant Antibody (clone DS3572AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7261 | Anti-TIMELESS Immunohistochemistry Kit | IHC |
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