Rabbit Anti-TIMM44 Recombinant Antibody (clone R04-7H8)
CAT#: VS3-FY1903
This R04-7H8 recombinant monoclonal antibody has been meticulously designed to target human TIMM44. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This TIMM44 antibody has been teted positive by western blot analysis of Timeless in MCF-7, Hela lysates and immunocytochemistry analysis of TIMM44 (green) in Hela using TIMM44 antibody.
Tested Data
WB
Figure 1 Anti-TIMM44 Antibody (VS3-FY1903) in WB.
The Timeless expression in MCF-7, Hela lysates were detected using the anti-TIMM44 by western blot assay.
ICC
Figure 2 Anti-TIMM44 Antibody (VS3-FY1903) in ICC.
Immunocytochemistry staining of TIMM44 (green) in Hela using the anti-TIMM44 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Recombinant human TIMM44 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TIMM44
- Species Reactivity
- Human
- Clone
- R04-7H8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 51 kDa, while the observed molecular weight stands at 45 kDa.
- Related Disease
- Developmental And Epileptic Encephalopathy 12 and Spinocerebellar Ataxia 28
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Translocase Of Inner Mitochondrial Membrane 44; TIM44; Translocase Of Inner Mitochondrial Membrane 44 Homolog (Yeast); Mitochondrial Import Inner Membrane Translocase Subunit TIM44; Translocase Of Inner Mitochondrial Membrane 44 Homolog; MIMT44
- Gene ID
- 10469
- UniProt ID
- O43615
- Sequence Similarities
- Belongs to the Tim44 family.
- Cellular Localization
- Membrane, Mitochondrion, Mitochondrion inner membrane
- Post Translation Modifications
- Ubiquitination at Lys240, Lys246, and Lys264 (NX_O43615 [NX_O43615-1])
Modification sites at PhosphoSitePlus (O43615)
Glycosylation from GlyGen (O43615) 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- NP_006342.2
- Function
- Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TIMM44"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7263 | Anti-TIMM44 Immunohistochemistry Kit | IHC |
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