Rabbit Anti-TJP3 Recombinant Antibody (clone R08-4E8)
CAT#: VS3-FY1942
This R08-4E8 recombinant monoclonal antibody is specifically engineered to target human TJP3. It cross-reacts with rat. This tight Junction protein ZO 3 antibody was used in western blot analysis of Zonula occludens protein 3 in MCF-7, Caco2 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46863/1137_C4_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53337/if_selected.jpg
Normal Tissue
Figure 3 Small intestine
High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53337/141944_A_5_2_val_selected.jpg
Normal Tissue
Figure 4 Duodenum.
High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13244/29828_A_7_2_val_selected.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53337/141944_A_6_6.jpg
Normal Tissue
Figure 6 Fallopian tube
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53337/141944_A_8_7.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53337/141944_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105289-TJP3
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WB
Figure 1 Anti-TJP3 Antibody (VS3-FY1942) in WB.
The Zonula occludens protein 3 expression in MCF-7, Caco2 lysates were detected using the anti-Tight Junction Protein ZO 3 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human zonule occludens protein 3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat TJP3
- Species Reactivity
- Human, Rat
- Clone
- R08-4E8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 101 kDa, while the observed molecular weight stands at 140 kDa.
- Related Disease
- Celiac Disease 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Tight Junction Protein 3; Zonula Occludens Protein 3; Zona Occludens Protein 3; ZO3; Tight Junction Protein 3 (Zona Occludens 3); Tight Junction Protein ZO-3; Zona Occludens 3; ZO-3
- Gene ID
- 27134
- UniProt ID
- O95049
- Sequence Similarities
- Belongs to the MAGUK family.
- Cellular Localization
- Cell junction, Cell membrane, Membrane, Nucleus, Tight junction
- Post Translation Modifications
- Phosphorylated (). (O95049-ZO3_HUMAN)
Modification sites at PhosphoSitePlus (O95049)
Modification sites at neXtProt (NX_O95049)
Glycosylation from GlyGen (O95049) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047294567.1; XP_054176526.1; NP_001254489.1; NP_001254490.1
- Function
- TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton.
The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Isotype Control
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- Product Name
Secondary Antibody
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- Product Name
See other products for "TJP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3578 | Rabbit Anti-TJP3 Recombinant Antibody (clone DS3578AB) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0248YJ | Rabbit Anti-TJP3 Polyclonal Antibody | ELISA, ICC, IF, WB, IP | Rabbit IgG |
| MRO-0249YJ | Rabbit Anti-TJP3 Recombinant Antibody (clone CBS192) | WB, IF | Rabbit IgG |
| VS13-YC1160 | Rabbit Anti-TJP3 Recombinant Antibody (VS13-YC1160) | WB | Rabbit IgG |
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