Rat Anti-TLR4 Recombinant Antibody (clone MTS510)
CAT#: MOR-0002-FY
This product is a recombinant Rat antibody that recognizes TLR4. The monoclonal Rat antibody MTS510 reacts with the epitope of TLR4.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/912_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/911_G5_4_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136869-TLR4
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Specifications
- Immunogen
- Ba/F3 cells expressing mouse TLR4 and MD-22.
- Host Species
- Rat
- Type
- Rat IgG2a
- Specificity
- Mouse TLR4
- Species Reactivity
- Mouse
- Clone
- MTS510
- Applications
- FC, FuncS, IF, IP
- Conjugate
- Unconjugated
- Related Disease
- Infectious diseases
Product Property
- Purification
- Protein G
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation.
Target
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Related Signaling Pathways
NLR Signaling Pathway
TLR Signaling Pathway
Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
- Product Name
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|---|---|---|---|
| TAB-613CL | Human Anti-TLR4 Recombinant Antibody (TAB-613CL) | FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-729 | Human Anti-TLR4 Recombinant Antibody (PABL-729) | Neut | Human IgG |
| HPAB-0318CQ | Rat Anti-Tlr4 Recombinant Antibody (HPAB-0318CQ) | FC, IP, Apop, IHC, WB, Inhib, FuncS | Rat IgG2a, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-722 | Human Anti-TLR4 Recombinant Antibody; Fab Fragment (PFBL-722) | Neut | Human Fab |
| HPAB-0318CQ-F(E) | Rat Anti-Tlr4 Recombinant Antibody Fab Fragment (HPAB-0318CQ-F(E)) | FC, IP, Apop | Rat Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-722 | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (PSBL-722) | Neut | Human scFv |
| HPAB-AP199-YC-S(P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (HPAB-AP199-YC-S(P)) | ELISA, FC, Inhib | Human scFv |
| HPAB-AP200-YC-S(P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (HPAB-AP200-YC-S(P)) | ELISA, FC, Inhib | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-181-S (P) | Human Anti-TLR4 Recombinant Antibody; scFv Fragment (PABX-181-S (P)) | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0425-FY30 | Human Anti-TLR4 scFv-Fc Chimera (VS-0425-FY30) | FC, ELISA, Neut | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC502 | Recombinant Anti-TLR4 Vesicular Antibody, EV Displayed (VS-0425-YC502) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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