Human Anti-TM4SF5 Recombinant Antibody (clone hEC2-C-1)
CAT#: FAMAB-0707WJ
This product is a humanized monoclonal antibody specific to TM4SF5 peptide. This antibody can be used in a variety of applications, such as ELISA, WB.
Published Data
Gene Expression
ELISA
Figure 1 Binding affinity of the humanized antibody for the cyclic peptide hTM4SF5EC2-C was analyzed using ELISA.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
SPR
Figure 2 Binding affinity for the cyclic peptide was analyzed using SPR system.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
WB
Figure 3 Whole cell protein lysates from HEK 293F-EV and HEK 293F-TM4SF5 cells were analyzed by western blotting with humanized anti-TM4SF5 antibody (hEC2-C-2) or anti-β-actin antibody.
Whole cell protein lysates from HEK 293F-EV and HEK 293F-TM4SF5 cells were immunoprecipitated with hEC2-C-2 antibody and analyzed by western blotting with the anti-Myc antibody.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
IF
Figure 4 CT-26 and HCT-116 cells were analyzed by confocal microscopy using normal human IgG and hEC2-C-2 antibody.
Scale bars, 20 μm.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
FuncS
Figure 5 Effects of the humanized anti-TM4SF5 monoclonal antibody on the migration of colon cancer cells
(A) Migration assay. Scale bars, 100 μm.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
FuncS
Figure 6 Effects of the humanized anti-TM4SF5 monoclonal antibody on the migration of colon cancer cells
Wound-healing assay. Scale bars, 300 μm. Migration properties of CT-26 and HCT-116 cells were compared after treatment with PBS, normal human IgG, or hEC2-C-2 antibody.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
IF
Figure 7 Effect of the humanized anti-TM4SF5 antibody on the expression of adhesion molecules in colon cancer cells
CT-26 and HCT-116 cells were treated with PBS, normal human IgG, or hEC2-C-2 antibody. Expression of E-cadherin and β-catenin was determined by western blotting with anti-E-cadherin and anti-β-catenin antibodies. (A) Confocal microscopy images after 3 h of treatment with DyLight 488-labeled human IgG control or humanized anti-TM4SF5 antibody (hEC2-C-2 Ab; 10 μg/mL). Scale bars, 20 μm.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
WB
Figure 8 Effect of the humanized anti-TM4SF5 antibody on the expression of adhesion molecules in colon cancer cells
CT-26 and HCT-116 cells were treated with PBS, normal human IgG, or hEC2-C-2 antibody. Expression of E-cadherin and β-catenin was determined by western blotting with anti-E-cadherin and anti-β-catenin antibodies. (B) Western blot analysis. β-actin was used as an internal control. The band intensities were measured and quantitative changes are shown on the graph.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
FuncS
Figure 9 Survival rate in the lung metastasis mouse model
CT-26 cells were intravenously injected into BALB/c mice. On day 5, PBS, human IgG, or hEC2-C-2 antibody was intravenously injected into the mice, and tumor growth was monitored after 22 days (PBS controls, n = 8; colon cancer cell-injected group, n = 12 each). (A) Experimental schedule. (B) Body weights were measured every other day for 20 days. (C) Survival of the humanized anti-TM4SF5 antibody-injected mice after implantation with CT-26 cells.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
FuncS
Figure 10 Inhibition of lung metastasis by the humanized anti-TM4SF5 monoclonal antibody
CT-26 cells were intravenously injected into BALB/c mice. On day 5, PBS, human IgG, or hEC2-C-2 antibody was intravenously injected into the mice, and tumor growth was monitored after 19 days (PBS controls, n = 8; colon cancer cell-injected group, n = 12 each). (A) Experimental schedule. (B) Macroscopic appearance of the lung. (C) Weight of the mouse lungs. **P < 0.01. (D) Histological examination of the lung tissues. Scale bars: 100X, 100 μm; 400X, 25 μm.
Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55674/1144_G10_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142484-TM4SF5
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Specifications
- Immunogen
- TM4SF5 peptide vaccine
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG
- Specificity
- Human TM4SF5
- Species Reactivity
- Human
- Clone
- hEC2-C-1
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Reliable For Western Blot Analysis
We recently incorporated this Anti-TM4SF5 recombinant antibody into our research workflow for Western Blot analysis. The product's high purity, stated as greater than 95% by SDS-PAGE, resulted in exceptionally clear bands with minimal background interference. This reliability has been crucial for our studies involving the tetraspanin family and signal transduction pathways.
Specific Humanized Antibody Performance
Our laboratory required a specific humanized monoclonal antibody for ELISA applications targeting the TM4SF5 peptide. This clone, hEC2-C-1, demonstrated excellent specificity and binding affinity in our assays. The consistency between different aliquots of this recombinant antibody has greatly improved the reproducibility of our data regarding cell surface glycoprotein expression levels.
Q&As
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What is the specific concentration of this antibody supplied in the vial?
A: The specific concentration for this anti-TM4SF5 recombinant antibody is not fixed for every batch and will be indicated on the vial label of the product you receive. We recommend checking the label upon receipt to ensure you use the correct concentration for your calculations and experimental dilutions.
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Can I store this antibody at 4°C for an extended period of time?
A: We recommend storing this product at +4°C only for the short term, specifically for 1-2 weeks. For long-term storage to maintain optimal stability and activity, you should aliquot the antibody and store it at -20°C. Please avoid repeated freeze/thaw cycles as they can degrade the protein.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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