Mouse Anti-TM4SF5 Recombinant Antibody (clone mEC2-C) (CAT#: FAMAB-0706WJ)

Figure 1 BALB/c mice were injected with PBS or hTM4SF5EC2-C peptide, along with Lipoplex(O) complex, three times at 10 day intervals (n = 5 per group).

The titers and the reactivity of the antibodies in the serum samples were measured by ELISA using the indicated peptides.

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.

Figure 2 Inhibition of lung metastasis by immunization with TM4SF5 cyclic peptide vaccine in a mouse model of colon cancer

BALB/c mice were injected with PBS, Lipoplex(O), or the complex o.htm4SF5EC2-C peptide and Lipoplex(O) at 10 day intervals (PBS controls, n = 8; colon cancer cell-injected group, n = 15). A metastasis model was established by intravenous implantation of CT-26 cells in the treated mice, and the body weight and survival rate of the mice was monitored. (A) Experimental schedule. (B) Body weights were measured every other day for 20 days after CT-26 cell implantation.

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.

Figure 3 Inhibition of lung metastasis by immunization with TM4SF5 cyclic peptide vaccine in a mouse model of colon cancer

BALB/c mice were injected with PBS, Lipoplex(O), or the complex o.htm4SF5EC2-C peptide and Lipoplex(O) at 10 day intervals (PBS controls, n = 8; colon cancer cell-injected group, n = 15). A metastasis model was established by intravenous implantation of CT-26 cells in the treated mice, and the body weight and survival rate of the mice was monitored. (C) Survival of the immunized mice after CT-26 cell implantation. (D) Macroscopic appearance of lungs examined at day 52. (E) Lung weight of the mice at day 52. **P < 0.01. (F) Histological examination of the lung tissues. Scale bars, 100 μm.

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.

Figure 4 Reduction of lung nodule numbers by immunization with a TM4SF5 peptide vaccine in a mouse model of colon cancer

BALB/c mice were injected with PBS or th.htm4SF5EC2-C peptide and Lipoplex(O) complex at 10 day intervals (n = 8 per group). The metastasis model was established as described in Figure ​Figure2, and the tumor growth was monitored until day 46 or 50. (A) Experimental schedule. (B) Macroscopic appearance of lungs and lung weight examined at day 46 (CT-26 group) and day 50 (PBS control group, Lipoplex(O) .htm4SF5EC2-C peptide/CT-26 group; n = 4 per group). (C) Number of lung nodules at day 46 (CT-26 group) and day 50 (PBS control group, Lipoplex(O) .htm4SF5EC2-C peptide/CT-26 group; n = 4 per group).

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.

Figure 5 Determination of monoclonal antibody binding affinity for the cyclic peptide hTM4SF5EC2-C using ELISA.

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.

Figure 6 Determination of binding affinity for the cyclic peptide hTM4SF5EC2-C using SPR system.

Biotinylated peptides were immobilized on a streptavidin chip, and increasing amounts of antibody were applied. Kinetic parameters are shown under the sensorgrams.

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.

Figure 7 Whole cell protein lysates from HEK 293F-EV (empty vector) and HEK 293F-TM4SF5 cells were analyzed by western blotting with anti-TM4SF5 monoclonal (mEC2-C), anti-Myc, or anti-β-actin antibodies.

Whole cell protein lysates from HEK 293F-EV and HEK 293F-TM4SF5 cells were immunoprecipitated with mEC2-C and analyzed by western blotting with the anti-Myc antibody.

Wu, G., Kim, D., Park, B. K., Park, S., Ha, J. H., Kim, T. H., ... & Lee, Y. (2016). Anti-metastatic effect of the TM4SF5-specific peptide vaccine and humanized monoclonal antibody on colon cancer in a mouse lung metastasis model. Oncotarget, 7(48), 79170.