Rabbit Anti-TNFRSF12A Recombinant Antibody (clone 7H8) (CAT#: ZG-0596U)

Rabbit Anti-TNFRSF12A Antibody (clone 7H8) displayed high specificity against the TNFRSF12A protein of Human.


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WB

Figure 1 Rabbit Anti-TNFRSF12A Antibody (ZG-0596U) in WB

Figure 1 Rabbit Anti-TNFRSF12A Antibody (ZG-0596U) in WB

Western Blot
Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate
All lanes: TNFRSF12A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14, 11 kD
Observed band size: 14 kDa

IHC

Figure 2 Rabbit Anti-TNFRSF12A Antibody (ZG-0596U) in IHC

Figure 2 Rabbit Anti-TNFRSF12A Antibody (ZG-0596U) in IHC

IHC image of ZG-0596U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human TWEAKR
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human TNFRSF12A
  • Species Reactivity
  • Human
  • Clone
  • 7H8
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Ileitis and Glioblastoma.

Product Property

  • Purification
  • Affinity chromatography purified
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Tweak-receptor; TweakR; CD antigen CD266; TNFRSF12A; FN14

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Immunotoxin

CAT Product Name Application Type
AGTO-G061G Anti-TNFRSF12A immunotoxin ITEM-4 (scFv)-Gel Cytotoxicity assay, Function study

Chimeric Antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-194LC Recombinant Anti-Human TNFRSF12A Antibody (Non-glycosylated) ELISA Humanized antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3610 Rabbit Anti-TNFRSF12A Recombinant Antibody (clone DS3610AB) IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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