Mouse Anti-TNFRSF8 Recombinant Antibody (clone Ber-H2) (CAT#: HPAB-0090-YJ)

This antibody is a recombinant mouse antibody that recognizes human CD30. Mab Ber-H2 could be applied in routinely processed, formaldehyde-fixed, paraffin-embedded tissue sections. Mab Ber-H2 can be used in the diagnostic for cancer. Using the Ber-H2 MoAb, these Ki-1 lymphomas could be easily distinguished from other nonlymphoid anaplastic large-cell tumors.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IF

Figure 1 30 μmtissue section was stained with a CD30 primary antibody (Ber-H2) and an Alexa 488-conjugated secondary antibody.

Figure 1 30 μmtissue section was stained with a CD30 primary antibody (Ber-H2) and an Alexa 488-conjugated secondary antibody.

Hansen, H. P., Engels, H. M., Dams, M., Paes Leme, A. F., Pauletti, B. A., Simhadri, V. L., ... & Schubert, R. (2014). Protrusion‐guided extracellular vesicles mediate CD30 trans‐signalling in the microenvironment of Hodgkin's lymphoma. The Journal of pathology, 232(4), 405-414.

FC

Figure 2 hESC line HES3 P37 (cultured in 20% FCS for 37 weeks) or HES3 p115 þ 12 (passaged 12 weeks in KOSR) were harvested for flow cytometric analysis with CD30 and TG30 antibodies.

Figure 2 hESC line HES3 P37 (cultured in 20% FCS for 37 weeks) or HES3 p115 þ 12 (passaged 12 weeks in KOSR) were harvested for flow cytometric analysis with CD30 and TG30 antibodies.

Chung, T. L., Turner, J. P., Thaker, N. Y., Kolle, G., Cooper‐White, J. J., Grimmond, S. M., ... & Wolvetang, E. J. (2010). Ascorbate promotes epigenetic activation of CD30 in human embryonic stem cells. Stem cells, 28(10), 1782-1793.

IF

Figure 3 Immunofluorescence microscopy of CD30 (green) in HES3 human embryonic stem cells cultured under 20% FCS medium with ascorbate (50 lg/ml).

Figure 3 Immunofluorescence microscopy of CD30 (green) in HES3 human embryonic stem cells cultured under 20% FCS medium with ascorbate (50 lg/ml).

Chung, T. L., Turner, J. P., Thaker, N. Y., Kolle, G., Cooper‐White, J. J., Grimmond, S. M., ... & Wolvetang, E. J. (2010). Ascorbate promotes epigenetic activation of CD30 in human embryonic stem cells. Stem cells, 28(10), 1782-1793.

IHC

Figure 4 TNFRSF8 (CD30) in embryonal carcinoma (main picture) and seminomas (inset).

Figure 4 TNFRSF8 (CD30) in embryonal carcinoma (main picture) and seminomas (inset).

BIERMANN, K., HEUKAMP, L. C., STEGER, K., ZHOU, H., FRANKE, F. E., SONNACK, V., ... & WANG-ECKERT, L. I. H. U. A. (2007). Genome-wide expression profiling reveals new insights into pathogenesis and progression of testicular germ cell tumors. Cancer Genomics-Proteomics, 4(5), 359-367.


Specifications

  • Immunogen
  • Co cells
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human TNFRSF8
  • Species Reactivity
  • Human
  • Clone
  • Ber-H2
  • Applications
  • IHC, IF, FC
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody Ber-H2 has been reported in applications of Immunohistochemistry, Immunofluorescence, Flow Cytometry. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • TNF Receptor Superfamily Member 8; Lymphocyte Activation Antigen CD30; CD30L Receptor; Ki-1 Antigen; D1S166E; CD30; Ber-H2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone Ber-H2"

See other products for "TNFRSF8"

Human Antibody

CAT Product Name Application Type
TAB-144 Anti-Human CD30 Recombinant Antibody (Iratumumab) ELISA, FC, IP, FuncS, IF, Neut, ICC IgG1 - kappa

Chimeric Antibody

CAT Product Name Application Type
TAB-153 Chimeric (Mouse/Human) Anti-TNFRSF8 Recombinant Antibody (clone Brentuximab) Neut, ELISA, IF, IP, FuncS, FC, ICC Human IgG1, κ

Immunotoxin

CAT Product Name Application Type
AGTO-G062E Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G062R Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA Cytotoxicity assay, Function study
AGTO-G062S Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-Sap Cytotoxicity assay, Function study
AGTO-L041E anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L041R anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-RTA Cytotoxicity assay, Functional assay

Humanized Antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-139LC Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) ELISA Human antibody

Low- or Non-fucosylated Oligosaccharide

CAT Product Name Application Type
Gly-139LC-1 Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0597MZ Chicken Anti-TNFRSF8 Polyclonal IgY WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-2165CQ Hamster Anti-Tnfrsf8 Recombinant Antibody (clone mCD30.1) FC, Stim, Costim, Block Hamster IgG1

Neutralizing Antibody

CAT Product Name Application Type
NEUT-2166CQ Hamster Anti-Tnfrsf8 Recombinant Antibody (clone 2SH12-5F) Neut, FC Hamster IgG1, κ

Rabbit Monoclonal Antibody

Recombinant Antibody

CAT Product Name Application Type
HPAB-0329-YC Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0329-YC) ELISA, FC, Cyt, FuncS, Inhib Mouse IgG
HPAB-0330-YC Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0330-YC) ELISA, FC, Cyt, FuncS, Inhib Chimeric (mouse/human) IgG1, κ
HPAB-0331-YC Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0331-YC) ELISA, FC, Cyt, FuncS, Inhib Mouse IgG
HPAB-0332-YC Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0332-YC) FC, Cyt, FuncS Human IgG1, κ
HPAB-0459-WJ Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0459-WJ) ELISA, FuncS Mouse IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-144 Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Iratumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-153 Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Brentuximab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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