Mouse Anti-TNFSF11 Recombinant Antibody (clone 8A7B9)
CAT#: VS7-0425-WR1023
This product is a mouse IgG1 antibody that binds to human TNFSF11. The antibody 8A7B9 recognizes specific amino acid domain of human TNFSF11 (AA: 74-308). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with monkey.
Gene Expression
Normal Tissue
Figure 1 High expression in lymph node
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45142/ihc_selected.jpg
Normal Tissue
Figure 2 Low expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45142/114242_B_7_5_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows moderate positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9193/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45142/114242_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HSkMC, NTERA-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120659-TNFSF11
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Specifications
- Immunogen
- Recombinant human TNFSF11 (AA: 74-308) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TNFSF11
- Species Reactivity
- Human, Monkey
- Clone
- 8A7B9
- Molecule Class
- Tumor Necrosis Factor
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Osteopetrosis, autosomal recessive 2 (OPTB2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:100 - 1:500
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
- Gene ID
- 8600
- UniProt ID
- O14788
- Long Name
- TNF superfamily member 11
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane; Secreted
- Post Translation Modifications
- Glycoprotein
- Protein Refseq
- NP_003692.1
- Function
- Cytokine; Developmental protein; Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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