Human Anti-TNFSF11 Recombinant Antibody (clone BA05-1) (CAT#: HPAB-0334-YJ)

Figure 1 The binding ability and AMG162 of BA05-1 and RANKL Quite, and the binding ability of BA05-2 and RANKL is slightly better than AMG162.

Figure 2 Ability of the three kinds of anti-RANKL antibody blocking RANKL of detection in conjunction with its receptor.

Specifically, it tests people RANK (2 μg/mL) are coated on 96 orifice plates by preceding 16 h.It is subsequently added into and various concentration 0.5 μg/ml biotin labeling RANKL of recombined human RANKL antibody BA05-1, BA05-2, AMG162 preincubate, 37 centigrate, 30 vmin. The Avidin of the diluted HRP label of 50 μl 1:5000 is added, 37 centigrate are incubated for the RANKL that 1 h is used to detect be coated in conjunction with RANK. Anti- RANKL BA05-1 and BA05-2 antibody specificity blocks the ability in conjunction with RANKL and RANK suitable with AMG162.

Figure 3 Detect anti-RANKL antibody BA05-1 and BA05-2 inhibit RANKL induction the differentiation of RAW osteoclast ability.

RAW264.7 cell and a certain amount of people RANKL (40 ng/ml) and different amounts of anti-RANKL antibody BA05-1, BA05- 2, AMG162 is incubated for 4 days altogether in cell culture medium. At the end of 4 days, anti-tartaric acid acid is carried out to cell using infiltration and acidification Then acid phosphatase (TRAP) vital staining handles 5 min with p-nitrophenyl phosphate (pNPP). It is molten that 50 μl 0.5 M NaOH are added Liquid terminates reaction. P-nitrophenol is converted by p-nitrophenyl phosphate using TRAP activity, passes through optical density at measurement 405nm. It is quantified. The results show that the osteoclast differentiation that BA05-1 and BA05-2 induces RANKL is inhibited, and with AMG162 has similar activity.